A comparative immunohistochemistry of O6-methylguanine-DNA methyltransferase and p53 in diffusely infiltrating astrocytomas

The DNA repair protein O6‐methylguanine–DNA methyltransferase (MGMT) removes mutagenic adducts from the O6 position of guanine, thereby protecting the genome against guanine : cytosine to adenine : thymine transition and, meanwhile, conferring tumor resistance to many anticancer alkylating agents co...

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Bibliographic Details
Published in:Neuropathology 2003-09, Vol.23 (3), p.203-209
Main Authors: Yuan, Qingguo, Matsumoto, Kenichi, Nakabeppu, Yusaku, Iwaki, Toru
Format: Article
Language:English
Subjects:
Adolescent
Adult
astrocytoma
Astrocytoma - metabolism
Astrocytoma - pathology
Cell Nucleus - metabolism
Central Nervous System Neoplasms - metabolism
Central Nervous System Neoplasms - pathology
Child
Child, Preschool
Cytoplasm - metabolism
Down-Regulation
Female
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Infant
Ki-67 Antigen - metabolism
Male
Middle Aged
O-Methylguanine-DNA Methyltransferase - biosynthesis
O-Methylguanine-DNA Methyltransferase - genetics
O6-methylguanine-DNA methyltransferase
p53
Tumor Suppressor Protein p53 - biosynthesis
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Summary:The DNA repair protein O6‐methylguanine–DNA methyltransferase (MGMT) removes mutagenic adducts from the O6 position of guanine, thereby protecting the genome against guanine : cytosine to adenine : thymine transition and, meanwhile, conferring tumor resistance to many anticancer alkylating agents commonly used in the treatment of malignant gliomas. Studies on the involvement of p53 protein in expression of the MGMT gene have provided conflicting results regarding the relation between p53 protein and MGMT gene expression. To examine the potential immunostaining pattern of MGMT expression and to evaluate the possible relationship between p53 and MGMT regulation, we assessed MGMT and p53 accumulation on 35 cases of diffusely infiltrating astrocytomas. With a few cases showing cytoplasmic staining, MGMT accumulation was mainly nuclear. The percentage of labeled tumor cells was lower in high‐grade astrocytomas than in low‐grade astrocytomas (P 
ISSN:0919-6544
1440-1789
DOI:10.1046/j.1440-1789.2003.00504.x