Toxicogenomic difference between diethylstilbestrol and 17beta-estradiol in mouse testicular gene expression by neonatal exposure

In this study, we investigated the effects of neonatal exposure to exogenous estrogen (diethylstilbestrol: DES, 17beta-estradiol: E2) on testicular gene expressions. Male C57BL/6J mice, 1 day after birth, were subcutaneously injected with DES or E2 (3 micrograms/mouse/day) for 5 days, and then they...

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Published in:Molecular reproduction and development 2004-01, Vol.67 (1), p.19
Main Authors: Adachi, Tetsuya, Koh, Kyu-Bom, Tainaka, Hitoshi, Matsuno, Yoshiharu, Ono, Yushin, Sakurai, Kenichi, Fukata, Hideki, Iguchi, Taisen, Komiyama, Masatoshi, Mori, Chisato
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Diethylstilbestrol - pharmacology
Estradiol - pharmacology
Estrogens, Non-Steroidal - pharmacology
Gene Expression Profiling
Gene Expression Regulation, Developmental
Male
Mice
Mice, Inbred C57BL
Oligonucleotide Array Sequence Analysis
Spermatozoa - cytology
Spermatozoa - metabolism
Testis - cytology
Testis - drug effects
Testis - physiology
Toxicogenetics
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Summary:In this study, we investigated the effects of neonatal exposure to exogenous estrogen (diethylstilbestrol: DES, 17beta-estradiol: E2) on testicular gene expressions. Male C57BL/6J mice, 1 day after birth, were subcutaneously injected with DES or E2 (3 micrograms/mouse/day) for 5 days, and then they were raised for 8 weeks. In morphological observation of 8-week-old mice testes, spermatozoa were absent from many seminiferous tubules in DES-treated mice testes, but there was no change in E2-treated mice testes. Analysis of in-house cDNA microarray (mouse cDNA 889 genes) revealed that 17 genes were altered in DES-treated mice testes at 8 weeks of age, compared to each control. Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) analysis of these genes revealed that some genes, which were changed in E2-treated testis, were the same as in DES-treated testis, whereas in other cases there was a difference between DES-treated and E2-treated testis. The present results suggest that each exogenous estrogenic compound has both a common gene expression change pattern and its own testicular gene expression change pattern. Mol. Reprod. Dev. 67: 19-25, 2004.
ISSN:1040-452X