Hypoglycemic effect and antioxidant potential of kaempferol-3,7-O-(alpha)-dirhamnoside from Bauhinia forficata leaves

• Published in: Journal of natural products (Washington, D.C.) D.C.), 2004-05, Vol.67 (5), p.829-832
• Main Authors: Sousa, E. de, Zanatta, L, Seifriz, I, Creczynski-Pasa, T.B, Pizzolatti, M.G, Szpoganicz, B, Silva, F.R.M.B
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bauhinia - chemistry; Biological and medical sciences; Biphenyl Compounds; Blood Glucose - drug effects; Brazil; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - drug therapy; Dose-Response Relationship, Drug; General and cellular metabolism. Vitamins; General pharmacology; Glucose Tolerance Test; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; Inhibitory Concentration 50; Kaempferols - chemistry; Kaempferols - isolation & purification; Kaempferols - pharmacology; Kaempferols - therapeutic use; Male; Medical sciences; Molecular Structure; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Picrates - pharmacology; Plant Leaves - chemistry; Plants, Medicinal - chemistry; Rats; Rats, Wistar
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np030513u

In vivo and in vitro treatments were carried out to investigate the effects of kaempferol-3,7-O-(alpha)-dirhamnoside (kaempferitrin), a major flavonoid compound of the n-butanol fraction from Bauhiniaforficata leaves, on serum glucose levels, as well as its antioxidant potential. Oral administration of kaempferitrin led to a significant hypoglycemic effect in normal and in alloxan-induced diabetic rats. In normal rats, blood glucose lowering was observed only with the higher dose of kaempferitrin (200 mg/kg) at 1 h after treatment. However, the hypoglycemic effect of kaempferitrin in diabetic rats was evident at all doses tested (50, 100, and 200 mg/kg), and this profile was maintained throughout the period studied for both higher doses. Additionally, in glucose-fed hyperglycemic normal rats, the kaempferitrin failed to decrease blood glucose levels. In vitro antioxidant properties or action against reactive oxygen species of this compound was also evaluated. The compound showed high reactivity with 1,1-diphenyl-2-picryl hydrazyl (DPPH), IC(50) of 84.0 +/- 7.8 microM, inhibited myeloperoxidase activity with K(0.5) = 86 +/- 9.9 microM, and decreased lipid peroxidation, induced by ascorbyl radical either in microsomes or in asolectin and phosphatidylcholine liposomes, with IC(50)'s of 320 +/- 14.1, 223 +/- 8.3, and 112 +/- 8.8 microM, respectively.