PCR Identification and Automated Ribotyping of Pseudomonas aeruginosa Clinical Isolates from Intensive Care Patients

Nosocomial isolates of Pseudomonas aeruginosa exhibit high rates of resistance to antibiotics, and are often multidrug resistant. P. aeruginosa clinical isolates (n=56) were obtained from ICU patients in a hospital in Pakistan over a 3-y period. Antimicrobial susceptibility of the 56 P. aeruginosa c...

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Bibliographic Details
Published in:Scandinavian journal of infectious diseases 2004-05, Vol.36 (5), p.342-349
Main Authors: Sarwari, Arif, Hasan, Rumina, Lim, Chuan-bian, Ng, Yvonne, Ng, Charis, Zaman, Sara
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Automation
Biological and medical sciences
Cross Infection - drug therapy
Cross Infection - microbiology
DNA, Bacterial - analysis
Female
Humans
Infectious diseases
Intensive Care Units
Male
Medical sciences
Microbial Sensitivity Tests
Polymerase Chain Reaction - methods
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - isolation & purification
Pseudomonas Infections - diagnosis
Ribotyping
Sampling Studies
Sensitivity and Specificity
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Summary:Nosocomial isolates of Pseudomonas aeruginosa exhibit high rates of resistance to antibiotics, and are often multidrug resistant. P. aeruginosa clinical isolates (n=56) were obtained from ICU patients in a hospital in Pakistan over a 3-y period. Antimicrobial susceptibility of the 56 P. aeruginosa clinical isolates was investigated using 7 antibiotics and the resistance rates were as follows: aztreonam (68% resistant), ceftazidime (67%), imipenem (66%), ofloxacin (59%), amikacin (56%), gentamicin (44%), and piperacillin-tazobactam (27%) (p
ISSN:0036-5548
1651-1980
DOI:10.1080/00365540410020109