Developmental regulation of claudin localization by fetal alveolar epithelial cells

Departments of 1 Physiology and 3 Pediatrics and 2 Institute for Environmental Medicine, University of Pennsylvania School of Medicine; 4 Division of Neonatology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104; and 5 Laboratory of Metabolism, National Cancer Institute...

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Published in:American journal of physiology. Lung cellular and molecular physiology 2004-12, Vol.287 (6), p.L1266-L1273
Main Authors: Daugherty, Brandy L, Mateescu, Madalina, Patel, Anand S, Wade, Kelly, Kimura, Shioko, Gonzales, Linda W, Guttentag, Susan, Ballard, Philip L, Koval, Michael
Format: Article
Language:English
Subjects:
1-Methyl-3-isobutylxanthine - pharmacology
8-Bromo Cyclic Adenosine Monophosphate - pharmacology
Dexamethasone - pharmacology
Endocytosis - drug effects
Endocytosis - physiology
Fetus
Gene Expression Regulation, Developmental
Humans
Lung - drug effects
Lung - embryology
Membrane Proteins - genetics
Sodium Chloride - pharmacology
Tight Junctions - physiology
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Summary:Departments of 1 Physiology and 3 Pediatrics and 2 Institute for Environmental Medicine, University of Pennsylvania School of Medicine; 4 Division of Neonatology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104; and 5 Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 Submitted 2 December 2003 ; accepted in final form 30 August 2004 Tight junction proteins in the claudin family regulate epithelial barrier function. We examined claudin expression by human fetal lung (HFL) alveolar epithelial cells cultured in medium containing dexamethasone, 8-bromo-cAMP, and isobutylmethylxanthanine (DCI), which promotes alveolar epithelial cell differentiation to a type II phenotype. At the protein level, HFL cells expressed claudin-1, claudin-3, claudin-4, claudin-5, claudin-7, and claudin-18, where levels of expression varied with culture conditions. DCI-treated differentiated HFL cells cultured on permeable supports formed tight transepithelial barriers, with transepithelial resistance (TER) >1,700 ohm/cm 2 . In contrast, HFL cells cultured in control medium without DCI did not form tight barriers (TER
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00423.2003