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Loss of Heterozygosity and Nonsense Mutation in Apc in Azoxymethane-induced Colonic Tumours in Min Mice
C57BL/6J Min/+ mice, which carry a nonsense mutation in Apc, were injected twice neonatally with 5 mg azoxymethane (AOM) /kg body weight. AOM treatment in comparison with untreated Min mice increased the incidence and number of colonic tumours from 6/14 to 22/24 (incidence) and 0.64±0.9 to 4.0±3.5...
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Published in: | Anticancer research 2004-09, Vol.24 (5A), p.2595-2599 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | C57BL/6J Min/+ mice, which carry a nonsense mutation in Apc, were injected twice neonatally with 5 mg azoxymethane (AOM) /kg
body weight. AOM treatment in comparison with untreated Min mice increased the incidence and number of colonic tumours from
6/14 to 22/24 (incidence) and 0.64±0.9 to 4.0±3.5 tumours per mice, respectively. Colonic tumours were analysed for loss of
heterozygosity (LOH) in Apc, and 32 of the samples showed LOH whereas 14 did not. In untreated Min mice, all 8 tumours had
LOH in Apc. All tumour samples from the AOM-treated Min mice were analysed for nonsense mutations between codons 686 and 1217
in the Apc gene, and one sample had a Gâ A transition mutation in codon 1047. No β-catenin mutations in the region coding
for phosphorylation sites important for degradation were found. In conclusion, the main mechanism for colonic tumour induction
in AOM-induced Min mice is LOH in Apc, but Apc nonsense mutations may also occur. |
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ISSN: | 0250-7005 1791-7530 |