Transformation of Escherichia coli mediated by natural phospholipids

Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-01, Vol.69 (1), p.235-237
Main Authors: Sato, Y. (Ibaraki Univ., Ami (Japan). Coll. of Agriculture), Kumazawa, N, Yoshikawa, K, Kurusu, Y
Format: Article
Language:English
Subjects:
Biological and medical sciences
CEPHALINS
DNA
DNA, Bacterial - genetics
ESCHERICHIA COLI
Escherichia coli - drug effects
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
GENETIC TRANSFORMATION
LECTINS
MAGNESIUM CHLORIDE
Phosphatidylethanolamines - pharmacology
PHOSPHOLIPIDS
plasmid
PLASMIDS
transformation
Transformation, Bacterial
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Summary:Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of MgCI2 or CaCI2 is essential. This method of transformation is applicable not only for small plasmid pHSG399 (2.3 Kb) but also for giant plasmid R6K (100 Kb).
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.69.235