Characterization of Symbiont Populations in Life-History Stages of Mussels from Chemosynthetic Environments

• Published in: The Biological bulletin (Lancaster) 2005-04, Vol.208 (2), p.145-155
• Main Authors: Salerno, Jennifer L., Macko, Stephen A., Hallam, Steve J., Bright, Monika, Won, Yong-Jin, McKiness, Zoe, Van Dover, Cindy L.
• Format: Article
• Language: English
• Subjects: Age Factors; Animals; Atlantic Ocean; Bacteria; Bacterial Physiological Phenomena; Bacteriocytes; Bathymodiolus azoricus; Bathymodiolus heckerae; Bivalvia - microbiology; Bivalvia - physiology; Bivalvia - ultrastructure; Colony Count, Microbial; Gills - ultrastructure; Hydrothermal vents; Isotope Labeling; Larvae; Marine; Marine biology; Methane; Methylococcaceae; Microscopy, Electron, Transmission; Mollusks; Mussels; Nitrogen; Physiological aspects; Species Specificity; Symbionts; Symbiosis; Symbiosis and Parasitology; Young animals
• ISSN: 0006-3185; 1939-8697
• DOI: 10.2307/3593123

The densities of chemoautotrophic and methanotrophic symbiont morphotypes were determined in life-history stages (post-larvae, juveniles, adults) of two species of mussels (Bathymodiolus azoricus and B. heckerae) from deep-sea chemosynthetic environments (the Lucky Strike hydrothermal vent and the Blake Ridge cold seep) in the Atlantic Ocean. Both symbiont morphotypes were observed in all specimens and in the same relative proportions, regardless of life-history stage. The relative abundance of symbiont morphotypes, determined by transmission electron microscopy, was different in the two species: chemoautotrophs were dominant (13:1-18:1) in B. azoricus from the vent site; methanotrophs were dominant (2:1-3:1) in B. heckerae from the seep site. The ratio of $CH_{4}:H_{2}S$ is proposed as a determinant of the relative abundance of symbiont types: where $CH_{4}:H_{2}S$ is less than 1, as at the Lucky Strike site, chemoautotrophic symbionts dominate; where $CH_{4}:H_{2}S$ is greater than 2, as at the seep site, methanotrophs dominate. Organic carbon and nitrogen isotopic compositions of B. azoricus ($\delta^{13}C = -30\textperthousand$; $\delta^{15}N = -9\textperthousand$) and B. heckerae ($\delta^{13}C = -56\textperthousand$; $\delta^{15}N = -2\textperthousand$) varied little among life-history stages and provided no record of a larval diet of photosynthetically derived organic material in the post-larval and juvenile stages.