Uptake of CdSe and CdSe/ZnS Quantum Dots into Bacteria via Purine-Dependent Mechanisms

Quantum dots (QDs) rendered water soluble for biological applications are usually passivated by several inorganic and/or organic layers in order to increase fluorescence yield. However, these coatings greatly increase the size of the particle, making uptake by microorganisms impossible. We find that...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2005-05, Vol.71 (5), p.2548-2557
Main Authors: Kloepfer, J. A, Mielke, R. E, Nadeau, J. L
Format: Article
Language:English
Subjects:
Adenine - metabolism
Adenosine Monophosphate - metabolism
Bacillus subtilis
Bacillus subtilis - metabolism
Bacteria
Bacteria - metabolism
Biological and medical sciences
Cadmium
Cells
Escherichia coli
Escherichia coli - metabolism
Fluorescence
Fundamental and applied biological sciences. Psychology
Light
Methods
Microbiology
Quantum Dots
Selenium
Signal transduction
Sulfides
Zinc Compounds
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Description
Summary:Quantum dots (QDs) rendered water soluble for biological applications are usually passivated by several inorganic and/or organic layers in order to increase fluorescence yield. However, these coatings greatly increase the size of the particle, making uptake by microorganisms impossible. We find that adenine- and AMP-conjugated QDs are able to label bacteria only if the particles are
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.71.5.2548-2557.2005