Corynebacterium sp. U-96 contains a cluster of genes of enzymes for the catabolism of sarcosine to pyruvate

The sarcosine oxidase gene and nearby genes from Corynebacterium sp. U-96 were determined. The genes for serine hydroxymethyltransferase, the Beta, delta, alpha, and gamma sub-units of sarcosine oxidase, serine dehydratase, and 10-formyItetrahydrofolate hydrolase are arranged in this order. This sug...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-05, Vol.69 (5), p.952-956
Main Authors: Suzuki, H. (Kitasato Univ., Tokyo (Japan)), Tamamura, R, Yajima, S, Kanno, M, Suguro, M
Format: Article
Language:English
Subjects:
Bacterial Proteins
Biological and medical sciences
CATABOLISM
Chromosome Mapping
Chromosomes, Bacterial
CORYNEBACTERIUM
Corynebacterium - enzymology
ENZYMES
ESCHERICHIA COLI
flavoprotein
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
gene organization
GENES
Genes, Bacterial
Multigene Family
NUCLEOTIDE SEQUENCE
PURIFICATION
PYRUVATES
Pyruvic Acid - metabolism
SARCOSINE
Sarcosine - metabolism
sarcosine oxidase
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Summary:The sarcosine oxidase gene and nearby genes from Corynebacterium sp. U-96 were determined. The genes for serine hydroxymethyltransferase, the Beta, delta, alpha, and gamma sub-units of sarcosine oxidase, serine dehydratase, and 10-formyItetrahydrofolate hydrolase are arranged in this order. This suggests that the bacteria contain a cluster of genes for the catabolism of sarcosine to pyruvate. The possibility that the gene cluster is a merit for the cellular energy demands of the bacteria is discussed. Functional expression of sarcosine oxidase in Escherichia coli was accomplished, but the Beta sub-unit and the Beta delta complex were expressed at a low level as a soluble protein.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.69.952