Fungal Types and Concentrations from Settled Dust in Normal Residences

• Published in: Journal of occupational and environmental hygiene 2005-10, Vol.2 (10), p.481-492
• Main Authors: Hicks, Jeffrey B., Lu, Elizabeth T., De Guzman, Rachel, Weingart, Michal
• Format: Article
• Language: English
• Subjects: Air Pollution, Indoor - analysis; carpet dust; Dust; Environmental Monitoring; Floors and Floorcoverings; fungal contamination; Fungi; Fungi - isolation & purification; Houses; Housing; Indoor air quality; mold; Reproducibility of Results; settled dust; Studies
• ISSN: 1545-9624; 1545-9632
• DOI: 10.1080/15459620500252860

Analysis of settled dust collected from carpeting and furnishings is occasionally used by investigators to determine whether an environment contains unusual fungi. Little information is available concerning the types and concentrations of culturable fungi present on textile surfaces in normal residential settings not affected by unusual mold reservoirs, such as from fungal growth sites within the built environment. This study presents the results of the collection and analysis of surface dust from 26 residential environments that were prescreened by interview, physical inspection, and air sampling to limit the surface dust collection to structures in which there was no history of water intrusion, flooding, plumbing leaks, signs of mold growth, or evidence of unusual airborne fungal spore types or concentrations. In those structures found to have no history or indications of water events or unusual fungi, surface dust was vacuumed from prescribed horizontal areas on carpet and textile-covered furnishings. These samples were then subjected to fungal culture, from which viable colonies were enumerated and identified. Based on the study results, it does not appear reasonable that the frequently quoted total fungi concentration exceeding 105 CFU/g is definitive evidence that a residential surface is contaminated with unusual amounts of culturable fungi. Collocated samples collected from eight side-by-side carpets sections revealed poor reproducibility. While settled dust sampling may be appropriate for determining the fungal status of a localized area, or as a gross screening tool, using settled dust results alone to establish the presence of unusual fungal types or concentrations within a structure appears to be inappropriate, and using settled dust results with other investigative methods, such as visual observations and air sampling, requires cautious interpretation.