Fast separation and sensitive detection of carcinogenic aromatic amines by reversed-phase micro-liquid chromatography coupled with electrochemical detection

A micro-LC method was developed for the fast and sensitive analysis of aromatic amines by electrochemical detection. The chromatographic separation of nine carcinogenic aromatic amines was performed on an ABZ + PLUS column with detection limits up to pM L(-1) levels. Mobile phase comprised of methan...

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Bibliographic Details
Published in:Journal of chromatography A 2005-09, Vol.1089 (1-2), p.52-58
Main Authors: Shelke, M, Sanghi, S.K, Asthana, A, Lamba, S, Sharma, M
Format: Article
Language:English
Subjects:
Amines - analysis
Amines - isolation & purification
aromatic amines
carcinogens
Carcinogens - analysis
Carcinogens - isolation & purification
chemical analysis
Chromatography, Liquid - methods
electrochemical detection
electrochemistry
Electrochemistry - methods
Food Additives - chemistry
food coloring
reversed-phase liquid chromatography
Sensitivity and Specificity
Water Pollutants, Chemical - analysis
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Summary:A micro-LC method was developed for the fast and sensitive analysis of aromatic amines by electrochemical detection. The chromatographic separation of nine carcinogenic aromatic amines was performed on an ABZ + PLUS column with detection limits up to pM L(-1) levels. Mobile phase comprised of methanol-acetate buffer of pH 5 (45:55, v/v) used at a flow rate of 0.2 ml min(-1). The detection was performed with a 6 mm glassy carbon electrode at an applied potential of 0.8 V versus Ag/AgCl. An intraday RSD for retention time and peak area were between 0.22% and 0.73% and 1.86% and 4.03%, respectively. The interdays RSD for retention time and peak area were between 0.47% and 1.35% and 2.04% and 4.42%, respectively. The applicability of the assay has been demonstrated by analyzing these aromatic amines in lake water and synthetic food colour additives. A comparison is given between electrochemical and UV detection.
ISSN:0021-9673