Cellular monitoring of the nuclear factor kappaB pathway for assessment of space environmental radiation

A screening assay for the detection of NF-kappaB-dependent gene induction using the destabilized variant of the reporter protein enhanced green fluorescent protein (d2EGFP) is used for assessing the biological effects of accelerated heavy ions as a model of space environmental radiation conditions....

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Bibliographic Details
Published in:Radiation research 2005-10, Vol.164 (4 Pt 2), p.527
Main Authors: Baumstark-Khan, Christa, Hellweg, Christine E, Arenz, Andrea, Meier, Matthias M
Format: Article
Language:English
Subjects:
Cell Line
Cosmic Radiation - adverse effects
DNA Damage
Dose-Response Relationship, Radiation
Heavy Ions - adverse effects
Humans
NF-kappa B - physiology
Signal Transduction - radiation effects
Transcription, Genetic - radiation effects
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Summary:A screening assay for the detection of NF-kappaB-dependent gene induction using the destabilized variant of the reporter protein enhanced green fluorescent protein (d2EGFP) is used for assessing the biological effects of accelerated heavy ions as a model of space environmental radiation conditions. The time course of d2EGFP expression and therefore of activation of NF-kappaB-dependent gene expression was measured after treatment with TNFA or after heavy-ion exposure using flow cytometry. The reported experiments clearly show that accelerated argon ions (95 MeV/nucleon, LET 230 keV/microm) induce the NF-kappaB pathway at low particle densities (1-2 particle hits per nucleus), which result in as few as 5-50 induced DSBs per cell.
ISSN:0033-7587