MIP-1beta, a novel biomarker for in vitro sensitization test using human monocytic cell line

In order to seek a novel biomarker for predicting skin sensitization, changes in the gene expression profile of THP-1 cells on exposure to 2,4-dinitrochlorobenzene (DNCB), p-phenylenediamine (pPD) and nickel sulfate (Ni) were assessed using oligo-DNA microarrays. While the change in gene expression...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology in vitro 2006-08, Vol.20 (5), p.736
Main Authors: Hirota, Morihiko, Moro, Osamu
Format: Article
Language:English
Subjects:
B7-2 Antigen - analysis
Biomarkers
Cathepsin B - genetics
Cell Line
Chemokine CCL4
Chemokines, CC - biosynthesis
Dinitrochlorobenzene - toxicity
Heme Oxygenase-1 - genetics
Humans
Interleukin-1 - genetics
Macrophage Inflammatory Proteins - biosynthesis
Monocytes - drug effects
Monocytes - metabolism
Nickel - toxicity
Oligonucleotide Array Sequence Analysis
Phenylenediamines - toxicity
Skin - drug effects
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to seek a novel biomarker for predicting skin sensitization, changes in the gene expression profile of THP-1 cells on exposure to 2,4-dinitrochlorobenzene (DNCB), p-phenylenediamine (pPD) and nickel sulfate (Ni) were assessed using oligo-DNA microarrays. While the change in gene expression varied depending on the sensitizers, up-regulation of MIP-1 beta mRNA expression was detected in both DNCB-treated and Ni-treated THP-1 cells. This finding was validated by RT-PCR and confirmed at the protein level by ELISA. Secretion of MIP-1 beta from THP-1 was detected after 24-h treatment with sensitizers such as DNCB, Ni, 2-mercaptobenzothiazole (2-MBT) and cobalt sulfate (Co), while pPD and non-sensitizers such as sodium dodecyl sulfate (SDS) and benzalkonium chloride (BC) had no effect. The use of both MIP-1 beta production and CD86 expression as criteria reduced the number of false-negatives, and the results were in good agreement with those of in vivo assays. MIP-1 beta may be useful as a novel biomarker in in vitro sensitization assay using THP-1 cells, either alone or in combination with known markers.
ISSN:0887-2333