Chloromethane, methyl donor in veratryl alcohol biosynthesis in Phanerochaete chrysosporium and other lignin-degrading fungi

Chloromethane, a gaseous natural product implicated in methylation processes in Phellinus pomaceus, has been shown to act as methyl donor in veratryl alcohol biosynthesis in the lignin-degrading fungi Phanerochaete chrysosporium, Phlebia radiata, and Coriolus versicolor, none of which released detec...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1990-11, Vol.56 (11), p.3450-3457
Main Authors: Harper, D.B. (The Queen's University of Belfast, Belfast, Northern Ireland), Buswell, J.A, Kennedy, J.T, Hamilton, J.T.G
Format: Article
Language:English
Subjects:
ALCOHOLES
ALCOOL
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
BIOSINTESIS
BIOSYNTHESE
Biotechnology
CORIOLUS VERSICOLOR
Fundamental and applied biological sciences. Psychology
METANO
METHANE
METHIONINE
METHYLATION
METILACION
METIONINA
Mission oriented research
PHANEROCHAETE
PHLEBIA
Phlebia radiata
Physiology and Biotechnology
Physiology and metabolism
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Summary:Chloromethane, a gaseous natural product implicated in methylation processes in Phellinus pomaceus, has been shown to act as methyl donor in veratryl alcohol biosynthesis in the lignin-degrading fungi Phanerochaete chrysosporium, Phlebia radiata, and Coriolus versicolor, none of which released detectable amounts of CH3Cl during growth. When P. chrysosporium was grown in a medium containing C2H3Cl, levels of C2H3 incorporation into the 3- and 4-O-methyl groups of veratryl alcohol were very high and initially similar to those observed when the medium was supplemented with L-[methyl-2H3]methionine. When C2H3Cl was added to cultures actively synthesizing veratryl alcohol, incorporation of C2H3 was very rapid, with 81% of veratryl alcohol labeled after 12 h. By contrast, incorporation of C2H3 from L-[methyl-2H3]methionine was comparatively slow, attaining 10% after 12 h. It is proposed that these lignin-degrading fungi possess a tightly channeled multienzyme system in which CH3Cl biosynthesis is closely coupled to CH3Cl utilization for methylation of veratryl alcohol precursors
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.56.11.3450-3457.1990