Identification of a Naegleria fowleri Membrane Protein Reactive with Anti-Human CD59 Antibody

Naegleria fowleri, the causative agent of primary amebic meningoencephalitis, is resistant to complement lysis. The presence of a complement regulatory protein on the surface of N. fowleri was investigated. Southern blot and Northern blot analyses demonstrated hybridization of a radiolabeled cDNA pr...

Full description

Saved in:
Bibliographic Details
Published in:Infection and Immunity 2006-02, Vol.74 (2), p.1189-1195
Main Authors: Fritzinger, Angela E, Toney, Denise M, MacLean, Rebecca C, Marciano-Cabral, Francine
Format: Article
Language:English
Subjects:
Amebiasis - parasitology
Animals
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - metabolism
Antibodies, Protozoan - genetics
Antibodies, Protozoan - immunology
Antibodies, Protozoan - metabolism
Biological and medical sciences
CD59 Antigens - genetics
CD59 Antigens - immunology
CD59 Antigens - metabolism
Cell Line
Complement C9
Cross Reactions
Fundamental and applied biological sciences. Psychology
Fungal and Parasitic Infections
Humans
Immunoprecipitation
Membrane Proteins - genetics
Membrane Proteins - immunology
Membrane Proteins - metabolism
Meningoencephalitis - parasitology
Microbiology
Naegleria fowleri
Naegleria fowleri - metabolism
Naegleria fowleri - pathogenicity
Protozoan Proteins - genetics
Protozoan Proteins - immunology
Protozoan Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Naegleria fowleri, the causative agent of primary amebic meningoencephalitis, is resistant to complement lysis. The presence of a complement regulatory protein on the surface of N. fowleri was investigated. Southern blot and Northern blot analyses demonstrated hybridization of a radiolabeled cDNA probe for CD59 to genomic DNA and RNA, respectively, from pathogenic N. fowleri. An 18-kDa immunoreactive protein was detected on the membrane of N. fowleri by Western immunoblot and immunofluorescence analyses with monoclonal antibodies for human CD59. Complement component C9 immunoprecipitated with the N. fowleri "CD59-like" protein from amebae incubated with normal human serum. In contrast, a gene or protein similar to CD59 was not detected in nonpathogenic, complement-sensitive N. gruberi amebae. Collectively, our studies suggest that a protein reactive with antibodies to human CD59 is present on the surface of N. fowleri amebae and may play a role in resistance to lysis by cytolytic proteins.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.74.2.1189-1195.2006