MyD88 Innate Immune Function in a Zebrafish Embryo Infection Model

Innate immunity signaling mechanisms during vertebrate embryogenesis are largely unknown. To study Toll-like receptor (TLR) signaling function in the zebrafish embryo model, we designed an experimental setup for antisense morpholino knockdown under conditions of bacterial infection. Clearance of Sal...

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Bibliographic Details
Published in:Infection and Immunity 2006-04, Vol.74 (4), p.2436-2441
Main Authors: Sar, Astrid M. van der, Stockhammer, Oliver W, Laan, Carina van der, Spaink, Herman P, Bitter, Wilbert, Meijer, Annemarie H
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - genetics
Adaptor Proteins, Signal Transducing - physiology
Animals
Bacterial Outer Membrane Proteins - biosynthesis
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - metabolism
Biological and medical sciences
Danio rerio
Disease Models, Animal
Fundamental and applied biological sciences. Psychology
Host Response and Inflammation
Immunity, Innate - genetics
Microbiology
Molecular Sequence Data
Myeloid Differentiation Factor 88 - genetics
Myeloid Differentiation Factor 88 - physiology
Oligonucleotides, Antisense - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Salmonella enterica
Salmonella Infections - genetics
Salmonella Infections - immunology
Salmonella typhimurium - genetics
Salmonella typhimurium - immunology
Signal Transduction - genetics
Signal Transduction - immunology
Toll-Like Receptors - genetics
Toll-Like Receptors - physiology
Zebrafish - embryology
Zebrafish - genetics
Zebrafish - immunology
Zebrafish Proteins - genetics
Zebrafish Proteins - physiology
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Summary:Innate immunity signaling mechanisms during vertebrate embryogenesis are largely unknown. To study Toll-like receptor (TLR) signaling function in the zebrafish embryo model, we designed an experimental setup for antisense morpholino knockdown under conditions of bacterial infection. Clearance of Salmonella enterica serovar Typhimurium Ra bacteria was significantly impaired after knockdown of myeloid differentiation factor 88 (MyD88), a common adaptor protein in TLR and interleukin-1 receptor signaling. Thereby, we demonstrate for the first time that the innate immune response of the developing embryo involves MyD88-dependent signaling, which further establishes the zebrafish embryo as a model for the study of vertebrate innate immunity.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.74.4.2436-2441.2006