Prestimulation of Head and Neck Cancer Cells with Growth Factors Enhances Treatment Efficacy

Objectives: In recent years, new chemotherapy regimens with promising activity, especially in first-line therapy (induction chemotherapy) of head and neck cancer (SCCHN), have been developed. Nevertheless, a major problem concerning the response of SCCHN to chemotherapy is the high percentage of res...

Full description

Saved in:
Bibliographic Details
Published in:Anticancer research 2006-03, Vol.26 (2A), p.1091-1095
Main Authors: HAMBEK, Markus, WERNER, Christian, BAGHI, Mehran, GSTÖTTNER, Wolfgang, KNECHT, Rainald
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Biological and medical sciences
Carcinoma, Squamous Cell - drug therapy
Carcinoma, Squamous Cell - pathology
Cell Cycle - drug effects
Cell Cycle - physiology
Cell Line, Tumor
Cisplatin - pharmacology
Drug Synergism
Epidermal Growth Factor - pharmacology
Granulocyte Colony-Stimulating Factor - pharmacology
Growth Substances - pharmacology
Head and Neck Neoplasms - drug therapy
Head and Neck Neoplasms - pathology
Humans
Interleukin-6 - pharmacology
Medical sciences
Otorhinolaryngology (head neck, general aspects and miscellaneous)
Otorhinolaryngology. Stomatology
Receptor, Epidermal Growth Factor - metabolism
Serotonin - pharmacology
Signal Transduction
STAT3 Transcription Factor - metabolism
Tumors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objectives: In recent years, new chemotherapy regimens with promising activity, especially in first-line therapy (induction chemotherapy) of head and neck cancer (SCCHN), have been developed. Nevertheless, a major problem concerning the response of SCCHN to chemotherapy is the high percentage of resting cells (G0-phase cells) being resistant to chemotherapy. To overcome this phenomenon, the capacity of several cytokines to switch on cells into the division cycle and progress to the chemosensitive phases (S-, M-phases) was investigated. Materials and Methods: Interleukin-6, serotonin, granulocyte colony stimulating factor (G-CSF) and epidermal growth factor (EGF) were used to stimulate G0-phase squamous cell cancer cells (Detroit 562, A431, UM-SCC 10B) for re-entry into the cell cycle to enhance the response to cisplatin. The proportion of G0-phase cells was detected through multicolor FACS analysis and Ki-67 staining. Results: Cell cycle re-entry was most effective after combination treatment with serotonin + EGF. The proportion of G0-phase cells was significantly reduced after stimulation with serotonin + EGF (p
ISSN:0250-7005
1791-7530