Proteomic analysis of differential protein expression in atherosclerosis

Abstract Although recent studies have shown that several pro-inflammatory proteins can be used as biomarkers for atherosclerosis, the mechanism of atherogenesis is unclear and little information is available regarding proteins involved in development of the disease. Atherosclerotic tissue samples we...

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Bibliographic Details
Published in:Biomarkers 2006-05, Vol.11 (3), p.279-290
Main Authors: Sung, H. J., Ryang, Y. S., Jang, S.-W., Lee, C. W., Han, K. H., Ko, J.
Format: Article
Language:English
Subjects:
Aortic Diseases
Atherosclerosis
Atherosclerosis (general aspects, experimental research)
Atherosclerosis - genetics
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
cardiovascular disease
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation
Humans
inflammation
Medical sciences
Proteins - analysis
Proteomics - methods
Signal Transduction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
two-dimensional electrophoresis
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Summary:Abstract Although recent studies have shown that several pro-inflammatory proteins can be used as biomarkers for atherosclerosis, the mechanism of atherogenesis is unclear and little information is available regarding proteins involved in development of the disease. Atherosclerotic tissue samples were collected from patients in order to identify the proteins involved in atherogenesis. The protein expression profile of atherosclerosis patients was analysed using two-dimensional electrophoresis-based proteomics. Thirty-nine proteins were detected that were differentially expressed in the atherosclerotic aorta compared with the normal aorta. Twenty-seven of these proteins were identified in the MS-FIT database. They are involved in a number of biological processes, including calcium-mediated processes, migration of vascular smooth muscle cells, matrix metalloproteinase activation and regulation of pro-inflammatory cytokines. Confirmation of differential protein expression was performed by Western blot analysis. Potential applications of the results include the identification and characterization of signalling pathways involved in atherogenesis, and further exploration of the role of selected identified proteins in atherosclerosis.
ISSN:1354-750X
1366-5804
DOI:10.1080/13547500500525458