The Transcriptome of Human Oocytes

The identification of genes and deduced pathways from the mature human oocyte can help us better understand oogenesis, folliculogenesis, fertilization, and embryonic development. Human metaphase II oocytes were used within minutes after removal from the ovary, and its transcriptome was compared with...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2006-09, Vol.103 (38), p.14027-14032
Main Authors: Kocabas, Arif Murat, Crosby, Javier, Ross, Pablo J., Otu, Hasan H., Beyhan, Zeki, Can, Handan, Tam, Wai-Leong, Rosa, Guilherme J. M., Halgren, Robert G., Lim, Bing, Fernandez, Emilio, Cibelli, Jose Bernardo
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Cell cycle
Chromatin
Complementary DNA
Datasets
Female
Females
Fertilization
Gene Expression Profiling
Gene Expression Regulation
Genes
Germ cells
Humans
Mice
Oligonucleotide Array Sequence Analysis
Oocytes
Oocytes - physiology
Reproducibility of Results
Reproductive system
Ribonucleic acid
RNA
Stem cells
Transcription, Genetic
Transcriptomes
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Summary:The identification of genes and deduced pathways from the mature human oocyte can help us better understand oogenesis, folliculogenesis, fertilization, and embryonic development. Human metaphase II oocytes were used within minutes after removal from the ovary, and its transcriptome was compared with a reference sample consisting of a mixture of total RNA from 10 different normal human tissues not including the ovary. RNA amplification was performed by using a unique protocol. Affymetrix Human Genome U133 Plus 2.0 GeneChip arrays were used for hybridizations. Compared with reference samples, there were 5,331 transcripts significantly up-regulated and 7,074 transcripts significantly down-regulated in the oocyte. Of the oocyte up-regulated probe sets, 1,430 have unknown function. A core group of 66 transcripts was identified by intersecting significantly up-regulated genes of the human oocyte with those from the mouse oocyte and from human and mouse embryonic stem cells. GeneChip array results were validated using RT-PCR in a selected set of oocyte-specific genes. Within the up-regulated probe sets, the top overrepresented categories were related to RNA and protein metabolism, followed by DNA metabolism and chromatin modification. This report provides a comprehensive expression baseline of genes expressed in in vivo matured human oocytes. Further understanding of the biological role of these genes may expand our knowledge on meiotic cell cycle, fertilization, chromatin remodeling, lineage commitment, pluripotency, tissue regeneration, and morphogenesis.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0603227103