Free radicals in exhaled breath condensate in cystic fibrosis and healthy subjects

Many markers of airway inflammation and oxidative stress can be measured non-invasively in exhaled breath condensate (EBC). However, no attempt has been made to directly detect free radicals using electron paramagnetic resonance (EPR) spectroscopy. Condensate was collected in 14 children with cystic...

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Bibliographic Details
Published in:Free radical research 2006-09, Vol.40 (9), p.901-909
Main Authors: Rosias, Philippe P.R., Den Hartog, Gertjan J.M., Robroeks, Charlotte M.H.H.T., Bast, AALT, Donckerwolcke, Raymond A.M.G., Heynens, Jan W.C.M, Suykerbuyk, Joelle, Hendriks, Han J.E, Jöbsis, Quirijn, Dompeling, Edward
Format: Article
Language:English
Subjects:
Adolescent
Adult
Breath Tests - methods
Catalase - chemistry
Child
Cyclic N-Oxides - chemistry
Cystic fibrosis
Cystic Fibrosis - metabolism
electron paramagnetic resonance
electron spin resonance
Electron Spin Resonance Spectroscopy - methods
Exhalation
exhaled breath condensate
Feasibility Studies
Female
free radicals
Free Radicals - analysis
Humans
Hydrogen Peroxide - chemistry
Male
reactive oxygen species
Reference Values
Reproducibility of Results
Sensitivity and Specificity
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Summary:Many markers of airway inflammation and oxidative stress can be measured non-invasively in exhaled breath condensate (EBC). However, no attempt has been made to directly detect free radicals using electron paramagnetic resonance (EPR) spectroscopy. Condensate was collected in 14 children with cystic fibrosis (CF) and seven healthy subjects. Free radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide. EPR spectra were recorded using a Bruker EMX® spectrometer. Secondly, to study the source of oxygen centered radical formation, catalase or hydrogen peroxide was added to the condensate. Radicals were detected in 18 out of 21 condensate samples. Analysis of spectra indicated that both oxygen and carbon centered radicals were trapped. Within-subject reproducibility was good in all but one subject. Quantitatively, there was a trend towards higher maximal peak heights of both oxygen and carbon centered radicals in the children with CF. Catalase completely suppressed the signals in condensate. Addition of hydrogen peroxide resulted in increased radical signal intensity. Detection of free radicals in EBC of children with CF and healthy subjects is feasible using EPR spectroscopy.
ISSN:1071-5762
1029-2470
DOI:10.1080/10715760500522648