Human cell engraftment after busulfan or irradiation conditioning of NOD/SCID mice

INSERM E217, Bordeaux cedex, France. Human hematopoietic stem cell (HSC) xenotransplantation in NOD/SCID mice requires recipient conditioning, classically achieved by sublethal irradiation. Pretreatment with immunosuppressive and alkylating agents has been reported, but has not been rigorously teste...

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Bibliographic Details
Published in:Haematologica (Roma) 2006-10, Vol.91 (10), p.1384-1387
Main Authors: Robert-Richard, E, Ged, C, Ortet, J, Santarelli, X, Lamrissi-Garcia, I, de Verneuil, H, Mazurier, F
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Busulfan - administration & dosage
Gamma Rays
Graft Rejection - drug therapy
Graft Rejection - prevention & control
Graft Rejection - radiotherapy
Hematologic and hematopoietic diseases
Humans
Medical sciences
Mice
Mice, Inbred NOD
Mice, SCID
Stem Cell Transplantation - methods
Transplantation Conditioning - methods
Transplantation, Heterologous - methods
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Summary:INSERM E217, Bordeaux cedex, France. Human hematopoietic stem cell (HSC) xenotransplantation in NOD/SCID mice requires recipient conditioning, classically achieved by sublethal irradiation. Pretreatment with immunosuppressive and alkylating agents has been reported, but has not been rigorously tested against standard irradiation protocols. Here, we report that treatment of mice with a single dose (35 mg/kg) of Busilvex, an injectable form of busulfan, enables equivalent engraftment compared to 3.5 Gy irradiation. Mice treated with two doses of 25 mg/kg to reduce busulfan toxicity showed increased chimerism. Busulfan conditioning and irradiation resulted in comparable sensitivity of HSC detection as evaluated by limiting dilution analysis.
ISSN:0390-6078
1592-8721