Microcapsules formulated in the enteric coating copolymer Eudragit L100 as delivery systems for oral vaccination against infections by gastrointestinal nematode parasites

Microcapsules using the copolymer of methacrylic acid (Eudragit L100) were formulated for oral delivery of vaccines against the enteral/parenteral nematode parasite Trichinella spiralis. Antigenic preparations from first stage larvae (L1) of T. spiralis were microencapsulated in Eudragit L100. The m...

Full description

Saved in:
Bibliographic Details
Published in:Journal of drug targeting 2006-09, Vol.14 (8), p.567-575
Main Authors: Dea-Ayuela, Maria A., Rama-Iñiguez, Sara, Torrado-Santiago, S., Bolas-Fernandez, F.
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Antigens, Helminth - administration & dosage
Antigens, Helminth - immunology
Biological and medical sciences
Capsules
Drug Delivery Systems
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Eudragit L100
Female
General pharmacology
Hydrogen-Ion Concentration
Immunoglobulin G - blood
Medical sciences
Mice
Microcapsules
nematodes
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Polymethacrylic Acids - chemistry
Th2 Cells - immunology
Trichinella spiralis
Trichinella spiralis - immunology
Trichinellosis - immunology
Trichinellosis - prevention & control
vaccine
Vaccines - administration & dosage
Vaccines - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Microcapsules using the copolymer of methacrylic acid (Eudragit L100) were formulated for oral delivery of vaccines against the enteral/parenteral nematode parasite Trichinella spiralis. Antigenic preparations from first stage larvae (L1) of T. spiralis were microencapsulated in Eudragit L100. The microcapsules prepared by the spray drying method were resistant to acid pH, although the antigen was rapidly released under neutral and basic environmental conditions. The native protein conformation and biological activity was preserved in the microcapsules, as assessed by SDS-PAGE and ELISA. When administered to NIH mice, the antigen loaded microcapsules protected against infection by T. spiralis at both the intestinal and muscular levels, the worm burden diminishing by 45.58 and 53.33%, respectively. Furthermore, following administration of the microparticles an increase of the serum IgG1 response, a marker for the Th2 type response, was evident. These results indicate that microcapsules formulated with anionic biocompatible polymers such as Eudragit may be useful for oral vaccination against nematode infections.
ISSN:1061-186X
1029-2330
DOI:10.1080/10611860600849464