Loading…
Spontaneous Recovery of Pathogenicity by Leishmania major hsp100⁻/⁻ Alters the Immune Response in Mice
By using repeated mouse infection cycles, we obtained an escape variant with restored infectivity and pathogenicity that originated from a single, noninfectious hsp100⁻/⁻ gene (formerly known as ΔclpB) replacement clone of Leishmania major, the causative agent of cutaneous leishmaniasis. This isolat...
Saved in:
Published in: | Infection and Immunity 2006-11, Vol.74 (11), p.6027-6036 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | By using repeated mouse infection cycles, we obtained an escape variant with restored infectivity and pathogenicity that originated from a single, noninfectious hsp100⁻/⁻ gene (formerly known as ΔclpB) replacement clone of Leishmania major, the causative agent of cutaneous leishmaniasis. This isolate elicited increased infiltration of immune cells to the site of infection and altered the polarization of the immune response in BALB/c mice from a predominantly TH2 type to a TH1 type. A clonal analysis resulted in isolation of two clones with antagonistic properties. While one clone exhibited restored infectivity in isolated macrophages but caused no persistent infection in the mouse model, the second clone was unable to infect macrophages in vitro but could establish a lasting infection and form progressive lesions in BALB/c mice. Our results add to the evidence that the TH1-TH2 dichotomy of the early immune response against L. major not only depends on the genetic predisposition of the host but also depends on intrinsic properties of the parasite. |
---|---|
ISSN: | 0019-9567 1098-5522 |
DOI: | 10.1128/IAI.00773-05 |