Gene expression in enhanced apoptosis of human lymphoma U937 cells treated with the combination of different free radical generators and hyperthermia

The effects of various free radicals derived from 6-formylpterin (6-FP), α-phenyl-tert-butyl nitrone (PBN) and 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDN...

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Bibliographic Details
Published in:Free radical research 2007, Vol.41 (1), p.73-84
Main Authors: Wada, Shigehito, Tabuchi, Yoshiaki, Kondo, Takashi, Cui, Zheng-Guo, Zhao, Qing-Li, Takasaki, Ichiro, Salunga, Thucydides L., Ogawa, Ryohei, Arai, Toshiyuki, Makino, Keisuke, Furuta, Isao
Format: Article
Language:English
Subjects:
Amidines - pharmacology
Apoptosis - physiology
Cell Line, Tumor
Cyclic N-Oxides - pharmacology
DNA microarray
Fever - metabolism
Fever - physiopathology
Free Radicals - metabolism
gene expression
Gene Expression - physiology
Gene Expression Profiling
heat stress
Humans
hyperthermia
Lymphoma - metabolism
Oligonucleotide Array Sequence Analysis
Oxidants - pharmacology
Oxidative stress
Oxidative Stress - physiology
Pterins - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
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Summary:The effects of various free radicals derived from 6-formylpterin (6-FP), α-phenyl-tert-butyl nitrone (PBN) and 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays containing approximately 16,600 genes and computational gene expression analysis tools. When the cells were treated for 10 min at 44°C (15% apoptosis level), 39 up-regulated and 3 down-regulated genes were identified. In the up-regulated genes, apoptosis- and unfolded protein response-associated genes were contained. The combined treatment with heat and either chemical enhanced apoptosis level (approximately 30%) and showed a chemical-specific gene expression pattern. Furthermore, the expression levels of selected genes were confirmed by a real-time quantitative PCR. The present results will provide a basis for further understanding the molecular mechanisms in enhancement of heat-induced apoptosis by different intracellular oxidative stress.
ISSN:1071-5762
1029-2470
DOI:10.1080/10715760600946432