Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes

Specific DNA probes based on variable regions V1 and V3 of 16S rRNA of lactic acid bacteria were designed. These probes were used in hybridization experiments with variable regions amplified by using the polymerase chain reaction. In this way, a rapid and sensitive method was developed for the ident...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1991-11, Vol.57 (11), p.3390-3393
Main Authors: Klijn, N. (Netherlands Institute for Dairy Research, Ede, The Netherlands), Weerkamp, A.H, Vos, W.M. de
Format: Article
Language:English
Subjects:
BACTERIAS ACIDOLACTICAS
BACTERIE LACTIQUE
Bacteriology
Base Sequence
BIOCHIMIE
Biological and medical sciences
BIOQUIMICA
DNA Probes
Fundamental and applied biological sciences. Psychology
IDENTIFICACION
IDENTIFICATION
Lactococcus - genetics
Lactococcus - isolation & purification
Leuconostoc
Microbiology
Molecular Sequence Data
Nucleic Acid Conformation
Polymerase Chain Reaction
RNA, Bacterial - chemistry
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Temperature
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Summary:Specific DNA probes based on variable regions V1 and V3 of 16S rRNA of lactic acid bacteria were designed. These probes were used in hybridization experiments with variable regions amplified by using the polymerase chain reaction. In this way, a rapid and sensitive method was developed for the identification and classification of Lactococcus and Leuconostoc species
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.57.11.3390-3393.1991