Development of a Reverse Genetics System Enabling the Rescue of Recombinant Avian Influenza Virus A/Turkey/England/50-92/91 (H5N1) (Desarrollo de un sistema de genetica reversa que permite el rescate del virus recombinante de influenza aviar A/Turkey/England/50-92/91 (H5N1))

We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This sy...

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Bibliographic Details
Published in:Avian diseases 2007-03, Vol.51 (1), p.393-395
Main Authors: Wedy Howard, Anna Hayman, Angie Lackenby, Alison Whiteley, Brandon Londt, Banks, Jill, McCauley, John, Wendy Barclay
Format: Article
Language:English
Subjects:
Animals
Cell Line
Complementary DNA
Cricetinae
DNA, Complementary
DNA, Viral
DNA-Directed RNA Polymerases - metabolism
Dogs
Genetic mutation
Genetic Techniques
H5N1 subtype influenza A virus
Human influenza
Humans
Influenza A virus
Influenza A Virus, H5N1 Subtype - genetics
Plasmids
Reporter genes
Research Note
Reverse genetics
RNA
Viruses
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Summary:We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans. /// Nosotros, describimos previamente la utilización de un sistema establecido de genética reversa para genérar virus recombinantes de influenza A humana a partir de moléculas clonadas de ADN complementario. En el presente trabajo hemos armado un conjunto de plásmidos que permiten la recuperación del virus H5N1 de influenza aviar A/Turkey/England/50-92/91 utilizando ADN complementario. Este sistema nos permite introducir mutaciones o deleciones en el ADN complementario para crear virus mutantes modificados específicamente en un gen seleccionado. Estos virus mutantes pueden entonces ser utilizados en futuros estudios de patogénesis en ayes y en estudios para comprender las restricciones de huésped de los virus de influenza aviar en humanos.
ISSN:0005-2086
1938-4351
DOI:10.1637/7645-050906r1.1