Neovascularization following traumatic brain injury: possible evidence for both angiogenesis and vasculogenesis

Objective: Our goal was to characterize the angiogenic response following traumatic brain injury (TBI). Methods: Western analysis for vascular endothelial growth factor (VEGF) expression, double immunofluorescence labeling of endothelium and vascular endothelial growth factor receptor 2 (VEGFR2), br...

Full description

Saved in:
Bibliographic Details
Published in:Neurological research (New York) 2007-06, Vol.29 (4), p.375-381
Main Authors: Morgan, Randy, Kreipke, Christian W., Roberts, George, Bagchi, Mihir, Rafols, José A.
Format: Article
Language:English
Subjects:
Animals
Brain Injuries - complications
Brain Injuries - metabolism
Brain Injuries - pathology
BRAIN TRAUMA
Bromodeoxyuridine - metabolism
Capillaries - pathology
CAPILLARY DENSITY
Disease Models, Animal
Male
Neovascularization, Pathologic - etiology
Neovascularization, Pathologic - metabolism
Neovascularization, Pathologic - pathology
Rats
Rats, Sprague-Dawley
Vascular Endothelial Growth Factor Receptor-2 - metabolism
VEGF
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective: Our goal was to characterize the angiogenic response following traumatic brain injury (TBI). Methods: Western analysis for vascular endothelial growth factor (VEGF) expression, double immunofluorescence labeling of endothelium and vascular endothelial growth factor receptor 2 (VEGFR2), bromodioxyuridine (BrdU) incorporation and measurement of capillary density, were all used to determine the temporal angiogenic response following TBI. Results: The angiogenic factors, VEGF and VEGFR2, increase following trauma. Capillary density increases and BrdU incorporation confirm the presence of newly formed vessels up to 48 hours post-injury. Discussion: Our results indicated that following TBI, there is a substantial increase in angiogenesis and based on morphologic characterization of BrdU-positive nuclei within the endothelium, we provide evidence for vasculogenesis following injury.
ISSN:0161-6412
1743-1328
DOI:10.1179/016164107X204693