Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay

A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixat...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1991-09, Vol.29 (9), p.2056-2058
Main Authors: Knowles, D.P. Jr. (Animal Disease Research Unit, USDA-ARS, Pullman, WA), Perryman, L.E, Kappmeyer, L.S, Hennager, S.G
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibodies, Protozoan - analysis
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
Babesia - immunology
BABESIA EQUI
Babesiosis - diagnosis
Babesiosis - immunology
Binding, Competitive
Biological and medical sciences
CABALLOS
CHEVAL
Complement Fixation Tests
DISTRIBUCION NATURAL
DISTRIBUTION NATURELLE
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Evaluation Studies as Topic
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Horse Diseases - diagnosis
Horse Diseases - immunology
Horses
LIQUIDE BIOLOGIQUE
LIQUIDOS CORPORALES
Molecular immunology
PROTEINAS
PROTEINE
Protozoan Proteins - immunology
PRUEBAS DE FIJACION DEL COMPLEMENTO
REACTION DE DEVIATION DU COMPLEMENT
Techniques
TEST ELISA
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Description
Summary:A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixation test results agreed in 94% (144) of the serum samples tested. The 10 discrepant serum samples were retested and analyzed for ability to immunoprecipitate in vitro translation products from B. equi merozoite mRNA. Five discrepant results were clearly resolved in favor of the CI ELISA, and the remaining five discrepancies were not definitively resolved
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.29.9.2056-2058.1991