Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus

SslI, a type II restriction endonuclease, as purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1991-12, Vol.57 (12), p.3677-3678
Main Authors: Benbadis, L. (Centre International de Recherche Daniel Carasso, France), Garel, J.R, Hartley, D.L
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
BACTERIOFAGOS
BACTERIOPHAGE
Biological and medical sciences
Calcium - pharmacology
Chromatography, Ion Exchange
Deoxyribonucleases, Type II Site-Specific - drug effects
Deoxyribonucleases, Type II Site-Specific - isolation & purification
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA
endonuclease
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Hydrolases
Magnesium - pharmacology
Manganese - pharmacology
NUCLEASAS
NUCLEASE
nucleotide sequence
Osmolar Concentration
phages
STREPTOCOCCUS
Streptococcus - enzymology
Substrate Specificity
Temperature
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Description
Summary:SslI, a type II restriction endonuclease, as purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.57.12.3677-3678.1991