Increased expression of mitochondrial glycerophosphate dehydrogenase and antioxidant enzymes in prostate cancer cell lines/cancer

The involvement of mitochondrial glycerophosphate dehydrogenase (mGPDH) has previously been established in the production of ROS in prostate cancer cell lines (LNCaP, DU145, PC3 and CL1). The current study demonstrates that the mRNA level of mGPDH in prostate cancer cells is 3.3-8.9-fold higher comp...

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Bibliographic Details
Published in:Free radical research 2007-01, Vol.41 (10), p.1116-1124
Main Authors: Roy Chowdhury, Subir K., Raha, Sandeep, Tarnopolsky, Mark A., Singh, Gurmit
Format: Article
Language:English
Subjects:
Animals
antioxidant enzymes
Antioxidants - chemistry
cancer
Cell Line, Tumor
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Glycerolphosphate Dehydrogenase - chemistry
Glycolysis
Humans
Liver - metabolism
Male
Mice
Mice, Inbred C57BL
Mitochondria - enzymology
Mitochondrial glycerophosphate dehydrogenase
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Rats
reactive oxygen species
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Summary:The involvement of mitochondrial glycerophosphate dehydrogenase (mGPDH) has previously been established in the production of ROS in prostate cancer cell lines (LNCaP, DU145, PC3 and CL1). The current study demonstrates that the mRNA level of mGPDH in prostate cancer cells is 3.3-8.9-fold higher compared to the normal prostate epithelial cell line, PNT1A. This is consistent with the enzymatic activity and protein level of mGPDH. However, cytochrome c oxidase (COX) activity is 2.9-3.2-fold down-regulated in androgen-independent prostate cancer cell lines. The level of antioxidant enzymes, catalase, MnSOD and CuZnSOD are up-regulated in prostate cancer cell lines. Furthermore, it was observed that the activity of mGPDH is significantly higher in liver tissues from all mice with cancer compared to liver tissues from control mice. These data suggest that the up-regulation of mGPDH, due to a highly glycolytic environment, contributes to the overall increase in ROS generation and may result in the progression of the cancer.
ISSN:1071-5762
1029-2470
DOI:10.1080/10715760701579314