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Biosilica-coated κ-carrageenan microspheres for yeast alcohol dehydrogenase encapsulation
In this study, a novel polysaccharide/inorganic hybrid biocomposite was prepared through biomineralization-inspired process for efficient immobilization of yeast alcohol dehydrogenase (YADH). YADH-encapuslated κ-carrageenan microspheres (KCM) were first coated with chitosan, which was used to guide...
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Published in: | Journal of biomaterials science. Polymer ed. 2007-01, Vol.18 (12), p.1517-1526 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | In this study, a novel polysaccharide/inorganic hybrid biocomposite was prepared through biomineralization-inspired process for efficient immobilization of yeast alcohol dehydrogenase (YADH). YADH-encapuslated κ-carrageenan microspheres (KCM) were first coated with chitosan,
which was used to guide and catalyze the biomimetic formation of silica, and then coated with silica derived from tetraethoxysiliane (TEOS). Scanning electron microscopy (SEM) equipped with a energy dispersive X-ray spectrometer (EDX) was employed to investigate the composition and thickness
of silica film. Compared with KCM, the silica-coated κ-carrageenan microspheres (SKCM) containing YADH exhibited improved anti-swelling and catalytic properties. Enzyme leakage of YADH in KCM was detected to be 63.9% after 1 h, while the enzyme leakage in SKCM was as low as 18.2%.
The KCM adsorbed water promptly and after 1 h maximum water uptake can be as high as 1576 wt%, while the water uptake of SKCM was only 143 wt% even after 48 h. The optimum pH and temperature for encapsulated YADH were pH 6.5 and 25°C, which were just the same as those for free YADH, but
the encapsulated YADH exhibited broader pH and temperature ranges for high activity. Furthermore, the relative activity of YADH in KCM declined almost to zero after 8 recycles, while the relative activity of YADH in SKCM still maintained more than 50%. The significantly increased recycling
stability of YADH in SKCM may be attributed to the effective inhibition of enzyme leakage by the compact biosilica layer. |
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ISSN: | 0920-5063 1568-5624 |
DOI: | 10.1163/156856207794761970 |