Effect of the pyridoindole antioxidant stobadine on ATP-utilisation by renal Na,K-ATPase in rats with streptozotocin-induced diabetes

The effect of the pyridoindole antioxidant stobadine on diabetes-induced changes of Na,K-ATPase, especially those concerning the utilisation of its substrate ATP, was investigated. Sixteen weeks of streptozotocin-induced diabetes (single i.v. dose of streptozotocin; 55 mg/kg) was followed by decreas...

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Published in:General physiology and biophysics 2007-09, Vol.26 (3), p.207
Main Authors: Vrbjar, N, Strelková, S, Javorková, V, Vlkovicová, J, Mézesová, L, Stefek, M, Kysel'ová, Z, Gajdosíková, A
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Antioxidants - administration & dosage
Carbolines - administration & dosage
Diabetes Mellitus, Experimental - chemically induced
Diabetes Mellitus, Experimental - metabolism
Dose-Response Relationship, Drug
Enzyme Activation - drug effects
Indoles - metabolism
Kidney - drug effects
Kidney - metabolism
Male
Rats
Rats, Wistar
Sodium-Potassium-Exchanging ATPase - metabolism
Streptozocin
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Summary:The effect of the pyridoindole antioxidant stobadine on diabetes-induced changes of Na,K-ATPase, especially those concerning the utilisation of its substrate ATP, was investigated. Sixteen weeks of streptozotocin-induced diabetes (single i.v. dose of streptozotocin; 55 mg/kg) was followed by decrease in the enzyme activity. This effect was emphasised in the presence of higher concentrations of substrate and in the presence of 8 mmol x l(-1) ATP it represented 20%. It might be a consequence of altered functional properties of Na,K-ATPase as suggested by 20% decrease in the V(max) value along with decrease in the K(m) value by 20%. Administration of 0.05% (w/w) stobadine in the diet to diabetic rats improved the function of renal Na,K-ATPase with respect to utilisation of ATP as suggested by significant increase in the enzyme activity in the whole concentration range of ATP investigated as a consequence of V(max) elevation to the level comparable to absolute controls. In conclusion, stobadine may play a positive role in restoring the functional properties of renal Na,K-ATPase, especially concerning the utilisation of energy derived from hydrolysis of ATP, improving thus the maintenance of ionic homeostasis during diabetes.
ISSN:0231-5882