Reconstructing specimens using DIC microscope images

Differential interference contrast (DIC) microscopy is a powerful visualization tool used to study live biological cells. Its use, however, has been limited to qualitative observations. The inherent nonlinear relationship between the object properties and the image intensity makes quantitative analy...

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Bibliographic Details
Published in:IEEE transactions on cybernetics 2003-10, Vol.33 (5), p.728-737
Main Authors: Kagalwala, F., Kanade, T.
Format: Article
Language:English
Subjects:
Biological cells
Biomedical optical imaging
Cybernetics
Image analysis
Image reconstruction
Interference
Mathematical models
Microscopy
Nonlinear optics
Nonlinearity
Optical properties
Optimization
Optimization methods
Quantitative analysis
Visualization
Volume measurement
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Summary:Differential interference contrast (DIC) microscopy is a powerful visualization tool used to study live biological cells. Its use, however, has been limited to qualitative observations. The inherent nonlinear relationship between the object properties and the image intensity makes quantitative analysis difficult. Toward quantitatively measuring optical properties of objects from DIC images, we develop a method to reconstruct the specimen's optical properties over a three-dimensional (3-D) volume. The method is a nonlinear optimization which uses hierarchical representations of the specimen and data. As a necessary tool, we have developed and validated a computational model for the DIC image formation process. We test our algorithm by reconstructing the optical properties of known specimens.
ISSN:1083-4419
2168-2267
1941-0492
2168-2275
DOI:10.1109/TSMCB.2003.816924