Assessment of the Genetic Diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans as a Basis To Identify Putative Pathogenicity Genes and a Type III Secretion System of the SPI-1 Family by Multiple Suppression Subtractive Hybridizations

Fluorescent amplified fragment length polymorphism revealed that strains of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans are genetically distinct and can be grouped into four genetic lineages. Four suppression subtractive hybridizations were then performed to isolate DN...

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Published in:Applied and Environmental Microbiology 2008-05, Vol.74 (10), p.3295-3301
Main Authors: Alavi, Seyed Mehdi, Sanjari, Saeideh, Durand, Fabien, Brin, Chrystelle, Manceau, Charles, Poussier, Stéphane
Format: Article
Language:English
Subjects:
Amplified fragment length polymorphism
Amplified Fragment Length Polymorphism Analysis
Bacterial Proteins - genetics
Biological and medical sciences
Cluster Analysis
Fluorescence in situ hybridization
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetic diversity
Genetic Variation
Genetics
Genotype
Life Sciences
Membrane Transport Proteins - genetics
Microbiology
Nucleic Acid Hybridization - methods
Pathogens
Phylogeny
Phytopathology and phytopharmacy
Plant Microbiology
Polymorphism
Vegetal Biology
Virulence Factors - genetics
Xanthomonas
Xanthomonas - genetics
Xanthomonas - pathogenicity
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Poussier, Stéphane
description Fluorescent amplified fragment length polymorphism revealed that strains of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans are genetically distinct and can be grouped into four genetic lineages. Four suppression subtractive hybridizations were then performed to isolate DNA fragments present in these bean pathogens and absent from closely related xanthomonads. Virulence gene candidates were identified such as homologs of hemagglutinins, TonB-dependent receptors, zinc-dependent metalloproteases, type III effectors, and type IV secretion system components. Unexpectedly, homologs of the type III secretion apparatus components (SPI-1 family), usually reported in animal pathogens and insect symbionts, were also detected.
doi_str_mv 10.1128/AEM.02507-07
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source American Society for Microbiology; PubMed Central
subjects Amplified fragment length polymorphism
Amplified Fragment Length Polymorphism Analysis
Bacterial Proteins - genetics
Biological and medical sciences
Cluster Analysis
Fluorescence in situ hybridization
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetic diversity
Genetic Variation
Genetics
Genotype
Life Sciences
Membrane Transport Proteins - genetics
Microbiology
Nucleic Acid Hybridization - methods
Pathogens
Phylogeny
Phytopathology and phytopharmacy
Plant Microbiology
Polymorphism
Vegetal Biology
Virulence Factors - genetics
Xanthomonas
Xanthomonas - genetics
Xanthomonas - pathogenicity
title Assessment of the Genetic Diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans as a Basis To Identify Putative Pathogenicity Genes and a Type III Secretion System of the SPI-1 Family by Multiple Suppression Subtractive Hybridizations
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