Biocompatibility studies of endothelial cells on a novel calcium phosphate/SiO2-xerogel composite for bone tissue engineering

The bone biomaterial BONITmatrix, a nanoporous, granular scaffold composed of hydroxylapatite, calcium phosphate and SiO2, linked by a dense collagen mesh, was tested for its biocompatibility using endothelial cells (EC) in the form of macrovascular HUVEC, microvascular HDMEC and the endothelial cel...

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Bibliographic Details
Published in:Biomedical materials (Bristol) 2008-03, Vol.3 (1), p.015007-015007
Main Authors: Thimm, Benjamin W, Unger, Ronald E, Neumann, Hans-Georg, Kirkpatrick, C James
Format: Article
Language:English
Subjects:
Bone Substitutes - chemistry
Calcium Phosphates - chemistry
Cell Proliferation
Cell Survival
Cells, Cultured
Endothelial Cells - cytology
Endothelial Cells - physiology
Gels - chemistry
Humans
Materials Testing
Silicon Dioxide - chemistry
Tissue Engineering - methods
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Summary:The bone biomaterial BONITmatrix, a nanoporous, granular scaffold composed of hydroxylapatite, calcium phosphate and SiO2, linked by a dense collagen mesh, was tested for its biocompatibility using endothelial cells (EC) in the form of macrovascular HUVEC, microvascular HDMEC and the endothelial cell line ISOHAS-1. Cells were examined for their adherence and growth on the biomaterial and this was followed by confocal laser scanning microscopy after vital staining or immunocytochemical reactions, as well as by scanning electron microscopy. Macro- and microvascular ECs predominantly spread on BONITmatrix-collagen mesh-covered surfaces and fibres and maintained their typical morphology. As ECs in vivo must build up a functional vasculature, the seeded cells were further tested for proinflammatory expression markers and cytokine expression after lipopolysaccharide stimulation. Protein-coating studies revealed that BONITmatrix-collagen scaffolds needed human blood serum coating to successfully support the growth of ECs. All cells expressed endothelium-specific surface marker proteins such as PECAM-1, VE-cadherin and vWF. The in vitro data support recent in vivo studies and indicate that this calcium phosphate/SiO2-xerogel composite could be a useful scaffold material for tissue engineering.
ISSN:1748-605X
1748-605X
DOI:10.1088/1748-6041/3/1/015007