Biocompatible chitosan-DNAzyme nanoparticle exhibits enhanced biological activity

DNAzymes are oligonucleotides capable of specific catalysis of target mRNA. To date, a delivery vehicle for DNAzymes has not been developed. Chitosan is a biomaterial obtained abundantly in nature. A biocompatible c-jun (an oncogene) DNAzyme nanoparticle formulated from chitosan was found to be more...

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Bibliographic Details
Published in:Journal of microencapsulation 2008-01, Vol.25 (6), p.421-425
Main Authors: Dass, Crispin R., Friedhuber, Anna M., Khachigian, Levon M., Dunstan, Dave E., Choong, Peter F. M.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biocompatible Materials - chemistry
Biological and medical sciences
Biotechnology - methods
bone
Bone and Bones - metabolism
c-jun
cancer
Chitosan
Chitosan - chemistry
deoxyribozyme
DNA, Catalytic - chemistry
Drug Delivery Systems
General pharmacology
Medical sciences
Mice
Mice, Inbred BALB C
nanoparticle
Nanoparticles - chemistry
Nanotechnology - methods
Osteosarcoma - metabolism
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Placebos
Proto-Oncogene Proteins c-jun - genetics
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Summary:DNAzymes are oligonucleotides capable of specific catalysis of target mRNA. To date, a delivery vehicle for DNAzymes has not been developed. Chitosan is a biomaterial obtained abundantly in nature. A biocompatible c-jun (an oncogene) DNAzyme nanoparticle formulated from chitosan was found to be more active against osteosarcoma (bone cancer) cells, inducing apoptotic cell death in these cells. The formulation was stable in serum for a week and at room temperature for a month. Clinically, knockdown of c-jun gene expression with chitosan nanobiotechnology may improve treatment outcome for tumours growing in bone.
ISSN:0265-2048
1464-5246
DOI:10.1080/02652040802033673