POLR2F, ATP6V0A1 and PRNP Expression in Colorectal Cancer: New Molecules with Prognostic Significance?

Background: DNA-directed RNA polymerase II subunit F (POLR2F), a subunit of the V0 domain of the vacuolar ATPase (ATP6V0A1) and the prion protein (PRNP) are molecules of potential importance in carcinogenesis and targeted cancer therapy. However, their expression has not been studied in colorectal c...

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Published in:Anticancer research 2008-03, Vol.28 (2B), p.1221
Main Authors: Antonacopoulou, Anna G, Grivas, Petros D, Skarlas, Lambros, Kalofonos, Melpomeni, Scopa, Chrisoula D, Kalofonos, Haralabos P
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Colorectal Neoplasms - enzymology
Colorectal Neoplasms - genetics
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - pathology
DNA-Directed RNA Polymerases - biosynthesis
DNA-Directed RNA Polymerases - genetics
Female
Gene Expression
Humans
Male
Middle Aged
Neoplasm Staging
Prion Proteins
Prions - biosynthesis
Prions - genetics
Prognosis
RNA Polymerase II - biosynthesis
RNA Polymerase II - genetics
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Vacuolar Proton-Translocating ATPases - biosynthesis
Vacuolar Proton-Translocating ATPases - genetics
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Summary:Background: DNA-directed RNA polymerase II subunit F (POLR2F), a subunit of the V0 domain of the vacuolar ATPase (ATP6V0A1) and the prion protein (PRNP) are molecules of potential importance in carcinogenesis and targeted cancer therapy. However, their expression has not been studied in colorectal carcinomas. Patients and Methods: Expression microarray data were analyzed using a novel computational tool to reveal elevated levels of POLR2F, ATP6V0A1 and PRNP in relapsed colorectal carcinoma patients. The mRNA levels of POLR2F, ATP6V0A1 and PRNP were evaluated by quantitative RT-PCR in 70 colorectal carcinomas and 17 normal tissue specimens and were correlated with clinicopathological parameters. Results: POLR2F and PRNP were up-regulated in colorectal carcinomas. Moreover, a significant difference in the expression levels of all three molecules between the right colon and the rectum was observed. High expression levels of POLR2F and ATP6V0A1 correlated with improved 3-year survival. Moreover, PRNP expression constituted an independent prognostic factor of the 3-year survival in multivariate analysis. Conclusion: POLR2F and PRNP exhibited elevated levels in carcinomas compared to normal tissue samples suggesting a possible role for these molecules in colorectal cancer. The association of the three molecules with survival or disease prognosis warrants further investigation.
ISSN:0250-7005
1791-7530