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POLR2F, ATP6V0A1 and PRNP Expression in Colorectal Cancer: New Molecules with Prognostic Significance?
Background: DNA-directed RNA polymerase II subunit F (POLR2F), a subunit of the V0 domain of the vacuolar ATPase (ATP6V0A1) and the prion protein (PRNP) are molecules of potential importance in carcinogenesis and targeted cancer therapy. However, their expression has not been studied in colorectal c...
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| Published in: | Anticancer research 2008-03, Vol.28 (2B), p.1221 |
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| container_title | Anticancer research |
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| creator | Antonacopoulou, Anna G Grivas, Petros D Skarlas, Lambros Kalofonos, Melpomeni Scopa, Chrisoula D Kalofonos, Haralabos P |
| description | Background: DNA-directed RNA polymerase II subunit F (POLR2F), a subunit of the V0 domain of the vacuolar ATPase (ATP6V0A1)
and the prion protein (PRNP) are molecules of potential importance in carcinogenesis and targeted cancer therapy. However,
their expression has not been studied in colorectal carcinomas. Patients and Methods: Expression microarray data were analyzed
using a novel computational tool to reveal elevated levels of POLR2F, ATP6V0A1 and PRNP in relapsed colorectal carcinoma patients.
The mRNA levels of POLR2F, ATP6V0A1 and PRNP were evaluated by quantitative RT-PCR in 70 colorectal carcinomas and 17 normal
tissue specimens and were correlated with clinicopathological parameters. Results: POLR2F and PRNP were up-regulated in colorectal
carcinomas. Moreover, a significant difference in the expression levels of all three molecules between the right colon and
the rectum was observed. High expression levels of POLR2F and ATP6V0A1 correlated with improved 3-year survival. Moreover,
PRNP expression constituted an independent prognostic factor of the 3-year survival in multivariate analysis. Conclusion:
POLR2F and PRNP exhibited elevated levels in carcinomas compared to normal tissue samples suggesting a possible role for these
molecules in colorectal cancer. The association of the three molecules with survival or disease prognosis warrants further
investigation. |
| format | article |
| fullrecord | <record><control><sourceid>pubmed_highw</sourceid><recordid>TN_cdi_pubmed_primary_18505059</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18505059</sourcerecordid><originalsourceid>FETCH-LOGICAL-h240t-7fe8eafb2e519e280669c96f2bb0aa022799e1904cfabbb5d12a98525f53f7833</originalsourceid><addsrcrecordid>eNo1z1FPwjAUBeDGaATRv2D65JNL2lu6tb4YXEBNEBZEX5d2tKymbKQdQf-9EDT34bx8OTn3DPVpJmmScUbOUZ8AJ0lGCO-hqxi_CElTKdgl6lHByeFkH9liPl3A5B6PlkX6SUYUq2aFi8WswOPvbTAxurbBrsF569tgqk55nKumMuEBz8wev7XeVDtvIt67rsZFaNdNGztX4Xe3bpx11RE_XqMLq3w0N385QB-T8TJ_Sabz59d8NE1qGJIuyawRRlkNhlNpQBwHVzK1oDVRigBkUhoqybCySmvNVxSUFBy45cxmgrEBuj31bnd6Y1blNriNCj_l_8MHcHcCtVvXexdMGTfK-wNnpQogSngqKQBlv2B5XmQ</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>POLR2F, ATP6V0A1 and PRNP Expression in Colorectal Cancer: New Molecules with Prognostic Significance?</title><source>EZB Electronic Journals Library</source><creator>Antonacopoulou, Anna G ; Grivas, Petros D ; Skarlas, Lambros ; Kalofonos, Melpomeni ; Scopa, Chrisoula D ; Kalofonos, Haralabos P</creator><creatorcontrib>Antonacopoulou, Anna G ; Grivas, Petros D ; Skarlas, Lambros ; Kalofonos, Melpomeni ; Scopa, Chrisoula D ; Kalofonos, Haralabos P</creatorcontrib><description>Background: DNA-directed RNA polymerase II subunit F (POLR2F), a subunit of the V0 domain of the vacuolar ATPase (ATP6V0A1)
and the prion protein (PRNP) are molecules of potential importance in carcinogenesis and targeted cancer therapy. However,
their expression has not been studied in colorectal carcinomas. Patients and Methods: Expression microarray data were analyzed
using a novel computational tool to reveal elevated levels of POLR2F, ATP6V0A1 and PRNP in relapsed colorectal carcinoma patients.
The mRNA levels of POLR2F, ATP6V0A1 and PRNP were evaluated by quantitative RT-PCR in 70 colorectal carcinomas and 17 normal
tissue specimens and were correlated with clinicopathological parameters. Results: POLR2F and PRNP were up-regulated in colorectal
carcinomas. Moreover, a significant difference in the expression levels of all three molecules between the right colon and
the rectum was observed. High expression levels of POLR2F and ATP6V0A1 correlated with improved 3-year survival. Moreover,
PRNP expression constituted an independent prognostic factor of the 3-year survival in multivariate analysis. Conclusion:
POLR2F and PRNP exhibited elevated levels in carcinomas compared to normal tissue samples suggesting a possible role for these
molecules in colorectal cancer. The association of the three molecules with survival or disease prognosis warrants further
investigation.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 18505059</identifier><language>eng</language><publisher>Greece: International Institute of Anticancer Research</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Colorectal Neoplasms - enzymology ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; DNA-Directed RNA Polymerases - biosynthesis ; DNA-Directed RNA Polymerases - genetics ; Female ; Gene Expression ; Humans ; Male ; Middle Aged ; Neoplasm Staging ; Prion Proteins ; Prions - biosynthesis ; Prions - genetics ; Prognosis ; RNA Polymerase II - biosynthesis ; RNA Polymerase II - genetics ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Vacuolar Proton-Translocating ATPases - biosynthesis ; Vacuolar Proton-Translocating ATPases - genetics</subject><ispartof>Anticancer research, 2008-03, Vol.28 (2B), p.1221</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18505059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Antonacopoulou, Anna G</creatorcontrib><creatorcontrib>Grivas, Petros D</creatorcontrib><creatorcontrib>Skarlas, Lambros</creatorcontrib><creatorcontrib>Kalofonos, Melpomeni</creatorcontrib><creatorcontrib>Scopa, Chrisoula D</creatorcontrib><creatorcontrib>Kalofonos, Haralabos P</creatorcontrib><title>POLR2F, ATP6V0A1 and PRNP Expression in Colorectal Cancer: New Molecules with Prognostic Significance?</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: DNA-directed RNA polymerase II subunit F (POLR2F), a subunit of the V0 domain of the vacuolar ATPase (ATP6V0A1)
and the prion protein (PRNP) are molecules of potential importance in carcinogenesis and targeted cancer therapy. However,
their expression has not been studied in colorectal carcinomas. Patients and Methods: Expression microarray data were analyzed
using a novel computational tool to reveal elevated levels of POLR2F, ATP6V0A1 and PRNP in relapsed colorectal carcinoma patients.
The mRNA levels of POLR2F, ATP6V0A1 and PRNP were evaluated by quantitative RT-PCR in 70 colorectal carcinomas and 17 normal
tissue specimens and were correlated with clinicopathological parameters. Results: POLR2F and PRNP were up-regulated in colorectal
carcinomas. Moreover, a significant difference in the expression levels of all three molecules between the right colon and
the rectum was observed. High expression levels of POLR2F and ATP6V0A1 correlated with improved 3-year survival. Moreover,
PRNP expression constituted an independent prognostic factor of the 3-year survival in multivariate analysis. Conclusion:
POLR2F and PRNP exhibited elevated levels in carcinomas compared to normal tissue samples suggesting a possible role for these
molecules in colorectal cancer. The association of the three molecules with survival or disease prognosis warrants further
investigation.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Colorectal Neoplasms - enzymology</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - pathology</subject><subject>DNA-Directed RNA Polymerases - biosynthesis</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>Prion Proteins</subject><subject>Prions - biosynthesis</subject><subject>Prions - genetics</subject><subject>Prognosis</subject><subject>RNA Polymerase II - biosynthesis</subject><subject>RNA Polymerase II - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Vacuolar Proton-Translocating ATPases - biosynthesis</subject><subject>Vacuolar Proton-Translocating ATPases - genetics</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNo1z1FPwjAUBeDGaATRv2D65JNL2lu6tb4YXEBNEBZEX5d2tKymbKQdQf-9EDT34bx8OTn3DPVpJmmScUbOUZ8AJ0lGCO-hqxi_CElTKdgl6lHByeFkH9liPl3A5B6PlkX6SUYUq2aFi8WswOPvbTAxurbBrsF569tgqk55nKumMuEBz8wev7XeVDtvIt67rsZFaNdNGztX4Xe3bpx11RE_XqMLq3w0N385QB-T8TJ_Sabz59d8NE1qGJIuyawRRlkNhlNpQBwHVzK1oDVRigBkUhoqybCySmvNVxSUFBy45cxmgrEBuj31bnd6Y1blNriNCj_l_8MHcHcCtVvXexdMGTfK-wNnpQogSngqKQBlv2B5XmQ</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Antonacopoulou, Anna G</creator><creator>Grivas, Petros D</creator><creator>Skarlas, Lambros</creator><creator>Kalofonos, Melpomeni</creator><creator>Scopa, Chrisoula D</creator><creator>Kalofonos, Haralabos P</creator><general>International Institute of Anticancer Research</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20080301</creationdate><title>POLR2F, ATP6V0A1 and PRNP Expression in Colorectal Cancer: New Molecules with Prognostic Significance?</title><author>Antonacopoulou, Anna G ; Grivas, Petros D ; Skarlas, Lambros ; Kalofonos, Melpomeni ; Scopa, Chrisoula D ; Kalofonos, Haralabos P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h240t-7fe8eafb2e519e280669c96f2bb0aa022799e1904cfabbb5d12a98525f53f7833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Colorectal Neoplasms - enzymology</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - pathology</topic><topic>DNA-Directed RNA Polymerases - biosynthesis</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Staging</topic><topic>Prion Proteins</topic><topic>Prions - biosynthesis</topic><topic>Prions - genetics</topic><topic>Prognosis</topic><topic>RNA Polymerase II - biosynthesis</topic><topic>RNA Polymerase II - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Vacuolar Proton-Translocating ATPases - biosynthesis</topic><topic>Vacuolar Proton-Translocating ATPases - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Antonacopoulou, Anna G</creatorcontrib><creatorcontrib>Grivas, Petros D</creatorcontrib><creatorcontrib>Skarlas, Lambros</creatorcontrib><creatorcontrib>Kalofonos, Melpomeni</creatorcontrib><creatorcontrib>Scopa, Chrisoula D</creatorcontrib><creatorcontrib>Kalofonos, Haralabos P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Antonacopoulou, Anna G</au><au>Grivas, Petros D</au><au>Skarlas, Lambros</au><au>Kalofonos, Melpomeni</au><au>Scopa, Chrisoula D</au><au>Kalofonos, Haralabos P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>POLR2F, ATP6V0A1 and PRNP Expression in Colorectal Cancer: New Molecules with Prognostic Significance?</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>28</volume><issue>2B</issue><spage>1221</spage><pages>1221-</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: DNA-directed RNA polymerase II subunit F (POLR2F), a subunit of the V0 domain of the vacuolar ATPase (ATP6V0A1)
and the prion protein (PRNP) are molecules of potential importance in carcinogenesis and targeted cancer therapy. However,
their expression has not been studied in colorectal carcinomas. Patients and Methods: Expression microarray data were analyzed
using a novel computational tool to reveal elevated levels of POLR2F, ATP6V0A1 and PRNP in relapsed colorectal carcinoma patients.
The mRNA levels of POLR2F, ATP6V0A1 and PRNP were evaluated by quantitative RT-PCR in 70 colorectal carcinomas and 17 normal
tissue specimens and were correlated with clinicopathological parameters. Results: POLR2F and PRNP were up-regulated in colorectal
carcinomas. Moreover, a significant difference in the expression levels of all three molecules between the right colon and
the rectum was observed. High expression levels of POLR2F and ATP6V0A1 correlated with improved 3-year survival. Moreover,
PRNP expression constituted an independent prognostic factor of the 3-year survival in multivariate analysis. Conclusion:
POLR2F and PRNP exhibited elevated levels in carcinomas compared to normal tissue samples suggesting a possible role for these
molecules in colorectal cancer. The association of the three molecules with survival or disease prognosis warrants further
investigation.</abstract><cop>Greece</cop><pub>International Institute of Anticancer Research</pub><pmid>18505059</pmid></addata></record> |
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| source | EZB Electronic Journals Library |
| subjects | Adult Aged Aged, 80 and over Colorectal Neoplasms - enzymology Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology DNA-Directed RNA Polymerases - biosynthesis DNA-Directed RNA Polymerases - genetics Female Gene Expression Humans Male Middle Aged Neoplasm Staging Prion Proteins Prions - biosynthesis Prions - genetics Prognosis RNA Polymerase II - biosynthesis RNA Polymerase II - genetics RNA, Messenger - biosynthesis RNA, Messenger - genetics Vacuolar Proton-Translocating ATPases - biosynthesis Vacuolar Proton-Translocating ATPases - genetics |
| title | POLR2F, ATP6V0A1 and PRNP Expression in Colorectal Cancer: New Molecules with Prognostic Significance? |
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