Enhanced Concentration-Dependent Cytotoxic Effect of the Dinuclear Copper(II) Complex of l-Carnitine [Cu2(l-carnitine)2Cl2(H2O)2]Cl2, Compared to l-Carnitine or Copper Chloride Dihydrate, in Human Leukemic Cell Lines

We studied the antitumor properties of the dinuclear copper(II) complex of l-carnitine [Cu2(l-carnitine)2Cl2(H2O)2]Cl2, as well as those of l-carnitine and copper chloride dihydrate, in human leukemic cells. The complex was synthesized and characterized using EPR, 1H NMR,13C NMR, IR, and UV−vis anal...

Full description

Saved in:
Bibliographic Details
Published in:Journal of medicinal chemistry 2008-07, Vol.51 (13), p.3713-3719
Main Authors: Thomadaki, Hellinida, Karaliota, Alexandra, Litos, Charalambos, Scorilas, Andreas
Format: Article
Language:English
Subjects:
Antineoplastic agents
Biological and medical sciences
Carnitine - chemistry
Carnitine - toxicity
Cell Line, Tumor
Cell Survival - drug effects
Chlorides - chemistry
Copper - chemistry
Electron Spin Resonance Spectroscopy
General aspects
Humans
Medical sciences
Molecular Structure
Pharmacology. Drug treatments
Water - chemistry
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We studied the antitumor properties of the dinuclear copper(II) complex of l-carnitine [Cu2(l-carnitine)2Cl2(H2O)2]Cl2, as well as those of l-carnitine and copper chloride dihydrate, in human leukemic cells. The complex was synthesized and characterized using EPR, 1H NMR,13C NMR, IR, and UV−vis analyses. Its cytotoxic effect on the human leukemia cell lines HL-60 and K562 was studied by assessing the metabolic activity of cells (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT method), the structural integrity of cell membrane using Trypan blue assay, and the proliferation capacity of cells studying growth curves. Both leukemia cell lines showed a concentration-specific increased cytotoxicity of the complex, compared to l-carnitine or copper chloride dihydrate, with distinct underlying mechanisms, which were decreased proliferation efficiency for HL-60 cells and increased necrotic phenomena for K562 cells. Our results are indicative of a concentration-specific enhanced antileukemic effect of the complex, implying its value as a tool in the implementation of leukemia.
ISSN:0022-2623
1520-4804
DOI:10.1021/jm701473x