Use of Fluorescence In Situ Hybridization for Rapid Identification of Staphylococci in Blood Culture Samples Collected in a Portuguese Hospital

Fluorescence in situ hybridization was used for the direct identification of staphylococci in blood cultures collected at a Portuguese hospital where staphylococci account for up to 35% of clinically relevant blood cultures. The assay was able to detect the presence/absence of staphylococci and dist...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2008-09, Vol.46 (9), p.3097-3100
Main Authors: Tavares, Ana, Inácio, João, Melo-Cristino, José, Couto, Isabel
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Cross Infection - diagnosis
Cross Infection - microbiology
Fundamental and applied biological sciences. Psychology
Humans
In Situ Hybridization, Fluorescence
Microbiology
Miscellaneous
Portugal
Sensitivity and Specificity
Staphylococcal Infections - diagnosis
Staphylococcal Infections - microbiology
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Summary:Fluorescence in situ hybridization was used for the direct identification of staphylococci in blood cultures collected at a Portuguese hospital where staphylococci account for up to 35% of clinically relevant blood cultures. The assay was able to detect the presence/absence of staphylococci and distinguish Staphylococcus aureus from coagulase-negative staphylococci in 4.5 h.
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.00910-08