HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer

Conclusion. GFP transgene was expressed in the lining cells of the perilymphatic space. Lentivirus vectors are safe and cause only minimal inflammatory reaction. Transgene products can be delivered into the perilymph by utilizing lentivirus vectors. Objectives. To analyze the efficiency and safety o...

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Bibliographic Details
Published in:Acta oto-laryngologica 2008-01, Vol.128 (6), p.613-620
Main Authors: Pietola, Laura, Aarnisalo, Antti A., Joensuu, Joonas, Pellinen, Riikka, Wahlfors, Jarmo, Jero, Jussi
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Biological and medical sciences
CD4
CD8-β
Cells, Cultured
Cochlea - metabolism
gene transfer
Gene Transfer Techniques
Genetic Vectors
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
inner ear
kanamycin
Kanamycin - pharmacology
Lentivirus
Lentivirus vector
Male
Medical sciences
Mice
Otorhinolaryngology. Stomatology
safety
Transduction, Genetic
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Summary:Conclusion. GFP transgene was expressed in the lining cells of the perilymphatic space. Lentivirus vectors are safe and cause only minimal inflammatory reaction. Transgene products can be delivered into the perilymph by utilizing lentivirus vectors. Objectives. To analyze the efficiency and safety of lentiviral vectors HOX-GFP and WOX-GFP in intracochlear gene transfer. Materials and methods. Lentivirus vectors were tested for their transduction efficiency in vivo in CD-1 mice. Half of the animals were pretreated with kanamycin. Lentivirus vector or saline (1 µl) was injected into the inner ear. All the animals were sacrificed 14 days after the surgery and the cochleae and selected organs were analyzed immunohistochemically. Results. HOX-GFP and WOX-GFP expression was restricted to the lining cells of the scala tympani and scala vestibuli. No GFP expression was seen in the organ of Corti or the spiral ganglion. Aminoglycoside treatment had no effect on the expression of these vectors. The distant spread of lentivirus vectors was minimal; only the liver of one animal showed some GFP expression. Inflammatory reaction caused by these vectors was mild. Few inflammatory cells were found in the perilymphatic space of the cochlea and in the vestibular organ.
ISSN:0001-6489
1651-2251
DOI:10.1080/00016480701663409