Polyethylene Gel in the Subcutaneous Tissue of Rats: Histopathologic and Systemic Evaluation

Purpose: To evaluate the histological and systemic response to subcutaneous injection of polyethylene gel in rats. Methods: Twenty-one white male rats were divided into 3 groups (G): G1 and G2 received subcutaneous polyethylene gel injection in the dorsal midline and were sacrificed at 30 and 60 pos...

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Bibliographic Details
Published in:Orbit (Amsterdam) 2008-01, Vol.27 (3), p.153-156
Main Authors: Schellini, Silvana A., Zimmermann, Gislaine P. M., Hoyama, Erika, Pellizon, Claudia H., Padovani, Carlos R., Selva, Dinesh
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials
Biopsy, Needle
Disease Models, Animal
Gels
histology
Immunohistochemistry
Injections, Subcutaneous
Male
Polyethylene
Polyethylene - administration & dosage
Polyethylene - adverse effects
Prostheses and Implants
Random Allocation
Rats
Rats, Wistar
Reference Values
Sensitivity and Specificity
Skin Absorption - drug effects
Skin Absorption - physiology
subcutaneous injections
Subcutaneous Tissue - drug effects
Subcutaneous Tissue - pathology
toxicity
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Summary:Purpose: To evaluate the histological and systemic response to subcutaneous injection of polyethylene gel in rats. Methods: Twenty-one white male rats were divided into 3 groups (G): G1 and G2 received subcutaneous polyethylene gel injection in the dorsal midline and were sacrificed at 30 and 60 postoperative days, respectively. G3 was not exposed to the polyethylene gel and was sacrificed after 60 days. Blood levels of lactate dehydrogenase (LDH), creatine kinase (CK), and alkaline phosphatase (ALP) were evaluated. The heart, kidney, liver, adrenal gland, injection site, and adjacent tissues were histologically examined. The results were submitted to statistical analysis. Results: There was no clinical evidence of extrusion, reduction of the injected volume, or abnormalities in the adjacent tissues. Blood levels of CK and LDH were normal and similar in all groups. ALP levels were significantly lower in G2 than in G1 and G3. The systemic organs were normal on histological examination in the 3 groups evaluated. Microscopically, the polyethylene gel was surrounded by a thin pseudocapsule formation and minimal inflammatory cell response, which decreased from G1 to G2. Conclusion: The subcutaneous injection of polyethylene gel in rats elicited minimal local inflammatory response and no systemic side effects.
ISSN:0167-6830
1744-5108
DOI:10.1080/01676830701555246