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Porcine Lung Surfactant Protein B Gene (SFTPB): cDNA Sequencing, Chromosomal Location, and Expression Studies
The porcine surfactant protein B ( SFTPB ) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 6...
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| Published in: | Animal biotechnology 2008-01, Vol.19 (3), p.127-137 |
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| container_title | Animal biotechnology |
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| creator | Cirera, S. Fredholm, M. |
| description | The porcine surfactant protein B (
SFTPB
) is a single copy gene on chromosome 3. Three different cDNAs for the
SFTPB
have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the
SFTPB
transcript. The expression level for the
SFTPB
gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the
SFTPB
in pigs with Actinobacillus pleuropneumoniae lung infection. |
| doi_str_mv | 10.1080/10495390801977584 |
| format | article |
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SFTPB
) is a single copy gene on chromosome 3. Three different cDNAs for the
SFTPB
have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the
SFTPB
transcript. The expression level for the
SFTPB
gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the
SFTPB
in pigs with Actinobacillus pleuropneumoniae lung infection.</description><identifier>ISSN: 1049-5398</identifier><identifier>EISSN: 1532-2378</identifier><identifier>DOI: 10.1080/10495390801977584</identifier><identifier>PMID: 18607785</identifier><language>eng</language><publisher>England: Taylor & Francis Group</publisher><subject>Actinobacillus Infections - genetics ; Actinobacillus Infections - metabolism ; Actinobacillus Infections - veterinary ; Actinobacillus pleuropneumoniae ; Alternative Splicing ; Amino Acid Sequence ; Animals ; Base Sequence ; Chromosomes, Mammalian - genetics ; DNA, Complementary - isolation & purification ; Gene Expression Regulation ; Lung - metabolism ; Lung - microbiology ; Molecular Sequence Data ; Physical Chromosome Mapping ; Pneumonia, Bacterial - genetics ; Pneumonia, Bacterial - metabolism ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Protein Isoforms - genetics ; Pulmonary Surfactant-Associated Protein B - genetics ; qPCR ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Deletion ; SFTPB ; SNP ; Sus crofa ; Sus scrofa - genetics ; Swine Diseases - genetics ; Swine Diseases - metabolism ; Swine Diseases - microbiology</subject><ispartof>Animal biotechnology, 2008-01, Vol.19 (3), p.127-137</ispartof><rights>Copyright Taylor & Francis Group, LLC 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-1c0274e5b4fa3f6529c41a8387d14c5fd847704336c664384edf000e43f4fa973</citedby><cites>FETCH-LOGICAL-c431t-1c0274e5b4fa3f6529c41a8387d14c5fd847704336c664384edf000e43f4fa973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18607785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cirera, S.</creatorcontrib><creatorcontrib>Fredholm, M.</creatorcontrib><title>Porcine Lung Surfactant Protein B Gene (SFTPB): cDNA Sequencing, Chromosomal Location, and Expression Studies</title><title>Animal biotechnology</title><addtitle>Anim Biotechnol</addtitle><description>The porcine surfactant protein B (
SFTPB
) is a single copy gene on chromosome 3. Three different cDNAs for the
SFTPB
have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the
SFTPB
transcript. The expression level for the
SFTPB
gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the
SFTPB
in pigs with Actinobacillus pleuropneumoniae lung infection.</description><subject>Actinobacillus Infections - genetics</subject><subject>Actinobacillus Infections - metabolism</subject><subject>Actinobacillus Infections - veterinary</subject><subject>Actinobacillus pleuropneumoniae</subject><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Chromosomes, Mammalian - genetics</subject><subject>DNA, Complementary - isolation & purification</subject><subject>Gene Expression Regulation</subject><subject>Lung - metabolism</subject><subject>Lung - microbiology</subject><subject>Molecular Sequence Data</subject><subject>Physical Chromosome Mapping</subject><subject>Pneumonia, Bacterial - genetics</subject><subject>Pneumonia, Bacterial - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Protein Isoforms - genetics</subject><subject>Pulmonary Surfactant-Associated Protein B - genetics</subject><subject>qPCR</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Deletion</subject><subject>SFTPB</subject><subject>SNP</subject><subject>Sus crofa</subject><subject>Sus scrofa - genetics</subject><subject>Swine Diseases - genetics</subject><subject>Swine Diseases - metabolism</subject><subject>Swine Diseases - microbiology</subject><issn>1049-5398</issn><issn>1532-2378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkU1LAzEQhoMoflR_gBfJSRRcTTbZTVa8aLUqFC1UzyFmE13ZTWqSxfbfG2nBg6CnTJjneRlmANjH6BQjjs4wolVBqlTiirGC0zWwjQuSZzlhfD3VqZ8lgG-BnRDeEcI5J2gTbGFeIsZ4sQ26ifOqsRqOe_sKp703UkVpI5x4F3Vj4RW81al9NB09Ta6Oz6G6friEU_3Ra5u81xM4fPOuc8F1soVjp2RsnD2B0tbwZj7zOoT0h9PY140Ou2DDyDbovdU7AM-jm6fhXTZ-vL0fXo4zRQmOGVYoZ1QXL9RIYsoirxTFkhPOakxVYWpOGUOUkFKVJSWc6toghDQlJhkVIwNwuMydeZcmDVF0TVC6baXVrg-irPKqIhj9C-YYFTxn3yBegsq7ELw2YuabTvqFwEh8H0P8OkZyDlbh_Uun6x9jtf0EXCyBxhrnO_npfFuLKBet88bLtOAgyF_57F_9lyXiPJIvYQCn2g</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Cirera, S.</creator><creator>Fredholm, M.</creator><general>Taylor & Francis Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080101</creationdate><title>Porcine Lung Surfactant Protein B Gene (SFTPB): cDNA Sequencing, Chromosomal Location, and Expression Studies</title><author>Cirera, S. ; Fredholm, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-1c0274e5b4fa3f6529c41a8387d14c5fd847704336c664384edf000e43f4fa973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Actinobacillus Infections - genetics</topic><topic>Actinobacillus Infections - metabolism</topic><topic>Actinobacillus Infections - veterinary</topic><topic>Actinobacillus pleuropneumoniae</topic><topic>Alternative Splicing</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Chromosomes, Mammalian - genetics</topic><topic>DNA, Complementary - isolation & purification</topic><topic>Gene Expression Regulation</topic><topic>Lung - metabolism</topic><topic>Lung - microbiology</topic><topic>Molecular Sequence Data</topic><topic>Physical Chromosome Mapping</topic><topic>Pneumonia, Bacterial - genetics</topic><topic>Pneumonia, Bacterial - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Protein Isoforms - genetics</topic><topic>Pulmonary Surfactant-Associated Protein B - genetics</topic><topic>qPCR</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Deletion</topic><topic>SFTPB</topic><topic>SNP</topic><topic>Sus crofa</topic><topic>Sus scrofa - genetics</topic><topic>Swine Diseases - genetics</topic><topic>Swine Diseases - metabolism</topic><topic>Swine Diseases - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cirera, S.</creatorcontrib><creatorcontrib>Fredholm, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Animal biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cirera, S.</au><au>Fredholm, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porcine Lung Surfactant Protein B Gene (SFTPB): cDNA Sequencing, Chromosomal Location, and Expression Studies</atitle><jtitle>Animal biotechnology</jtitle><addtitle>Anim Biotechnol</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>19</volume><issue>3</issue><spage>127</spage><epage>137</epage><pages>127-137</pages><issn>1049-5398</issn><eissn>1532-2378</eissn><abstract>The porcine surfactant protein B (
SFTPB
) is a single copy gene on chromosome 3. Three different cDNAs for the
SFTPB
have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the
SFTPB
transcript. The expression level for the
SFTPB
gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the
SFTPB
in pigs with Actinobacillus pleuropneumoniae lung infection.</abstract><cop>England</cop><pub>Taylor & Francis Group</pub><pmid>18607785</pmid><doi>10.1080/10495390801977584</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 1049-5398 |
| ispartof | Animal biotechnology, 2008-01, Vol.19 (3), p.127-137 |
| issn | 1049-5398 1532-2378 |
| language | eng |
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| source | Taylor and Francis Science and Technology Collection |
| subjects | Actinobacillus Infections - genetics Actinobacillus Infections - metabolism Actinobacillus Infections - veterinary Actinobacillus pleuropneumoniae Alternative Splicing Amino Acid Sequence Animals Base Sequence Chromosomes, Mammalian - genetics DNA, Complementary - isolation & purification Gene Expression Regulation Lung - metabolism Lung - microbiology Molecular Sequence Data Physical Chromosome Mapping Pneumonia, Bacterial - genetics Pneumonia, Bacterial - metabolism Polymerase Chain Reaction Polymorphism, Single Nucleotide Protein Isoforms - genetics Pulmonary Surfactant-Associated Protein B - genetics qPCR Sequence Alignment Sequence Analysis, DNA Sequence Deletion SFTPB SNP Sus crofa Sus scrofa - genetics Swine Diseases - genetics Swine Diseases - metabolism Swine Diseases - microbiology |
| title | Porcine Lung Surfactant Protein B Gene (SFTPB): cDNA Sequencing, Chromosomal Location, and Expression Studies |
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