effect of particle design on cellular internalization pathways

The interaction of particles with cells is known to be strongly influenced by particle size, but little is known about the interdependent role that size, shape, and surface chemistry have on cellular internalization and intracellular trafficking. We report on the internalization of specially designe...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2008-08, Vol.105 (33), p.11613-11618
Main Authors: Gratton, Stephanie E.A, Ropp, Patricia A, Pohlhaus, Patrick D, Luft, J. Christopher, Madden, Victoria J, Napier, Mary E, DeSimone, Joseph M
Format: Article
Language:English
Subjects:
Biological Sciences
Cell lines
Cell Membrane - metabolism
Cell membranes
Cells
Composite particles
Endocytosis
HeLa Cells
Humans
Internalization
Microelectromechanical systems
Microscopy
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanoparticles
Nanoparticles - ultrastructure
Particle interactions
Particle Size
Physical Sciences
Signal transduction
Surface chemistry
Transmission electron microscopy
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Summary:The interaction of particles with cells is known to be strongly influenced by particle size, but little is known about the interdependent role that size, shape, and surface chemistry have on cellular internalization and intracellular trafficking. We report on the internalization of specially designed, monodisperse hydrogel particles into HeLa cells as a function of size, shape, and surface charge. We employ a top-down particle fabrication technique called PRINT that is able to generate uniform populations of organic micro- and nanoparticles with complete control of size, shape, and surface chemistry. Evidence of particle internalization was obtained by using conventional biological techniques and transmission electron microscopy. These findings suggest that HeLa cells readily internalize nonspherical particles with dimensions as large as 3 μm by using several different mechanisms of endocytosis. Moreover, it was found that rod-like particles enjoy an appreciable advantage when it comes to internalization rates, reminiscent of the advantage that many rod-like bacteria have for internalization in nonphagocytic cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0801763105