Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates

porB DNA sequence analysis and Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) methods were compared for their abilities to discriminate strains and to identify epidemiologically congruent pairs of N. gonorrhoeae. Both methods provided high-level discrimination of strains. NG-MAST furth...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2009-02, Vol.47 (2), p.489-491
Main Authors: Liao, Mingmin, Helgeson, Sarah, Gu, Wei-Ming, Yang, Yang, Jolly, Ann M, Dillon, Jo-Anne R
Format: Article
Language:English
Subjects:
Antigens, Bacterial - genetics
Bacterial Typing Techniques - methods
Bacteriology
Biological and medical sciences
Cluster Analysis
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Female
Fundamental and applied biological sciences. Psychology
Genotype
Gonorrhea - epidemiology
Gonorrhea - microbiology
Humans
Male
Microbiology
Miscellaneous
Molecular Epidemiology - methods
Molecular Sequence Data
Neisseria gonorrhoeae
Neisseria gonorrhoeae - classification
Neisseria gonorrhoeae - genetics
Neisseria gonorrhoeae - isolation & purification
Porins - genetics
Sequence Analysis
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Summary:porB DNA sequence analysis and Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) methods were compared for their abilities to discriminate strains and to identify epidemiologically congruent pairs of N. gonorrhoeae. Both methods provided high-level discrimination of strains. NG-MAST further differentiated large porB-based clusters. However, considerations of cost suggest that porB DNA sequence analysis is a useful tool for preliminary molecular analysis of the epidemiology of N. gonorrhoeae.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01612-08