Evaluation of an Immunochromatographic Assay Kit for Rapid Identification of Mycobacterium tuberculosis Complex in Clinical Isolates

We evaluated a new immunochromatographic assay (ICA) using mouse monoclonal anti-MPT64 antibody for rapid discrimination between Mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. A study with mycobacteria and other organisms showed excellent sensitivity ([congruent wit...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2009-02, Vol.47 (2), p.481-484
Main Authors: Park, Mi Young, Kim, Young Jin, Hwang, Sang Hyun, Kim, Hyoung Hoi, Lee, Eun Yup, Jeong, Seok Hoon, Chang, Chulhun L
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Bacteriological Techniques - methods
Bacteriology
Biological and medical sciences
Chromatography, Affinity - methods
Fundamental and applied biological sciences. Psychology
Humans
Immunoassay
Mice
Microbiology
Miscellaneous
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium tuberculosis
Mycobacterium tuberculosis - isolation & purification
Sensitivity and Specificity
Tuberculosis - diagnosis
Tuberculosis - microbiology
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Summary:We evaluated a new immunochromatographic assay (ICA) using mouse monoclonal anti-MPT64 antibody for rapid discrimination between Mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. A study with mycobacteria and other organisms showed excellent sensitivity ([congruent with]99%) and specificity (100%) and an appropriate detection limit (10⁵ CFU/ml) when tested with M. tuberculosis H37Rv. This ICA can simplify the identification of M. tuberculosis in clinical laboratories.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01253-08