Identification of sequence elements regulating promoter activity and replication of a monopartite begomovirus-associated DNA β satellite

DNA β is a circular single-stranded satellite DNA associated with certain monopartite begomoviruses (family Geminiviridae) which causes economically important diseases such as cotton leaf curl disease. DNA β contains a single gene, βC1, which encodes a pathogenicity protein responsible for symptom p...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 2009, Vol.90 (Pt 1), p.253-260
Main Authors: Eini, Omid, Behjatnia, S.A. Akbar, Dogra, Satish, Dry, Ian B, Randles, John W, Rezaian, M. Ali
Format: Article
Language:English
Subjects:
Artificial Gene Fusion
Base Sequence
Begomovirus
Begomovirus - physiology
beta-glucuronidase
Binding Sites
Cotton leaf curl Multan virus
DNA Replication
DNA, Satellite - biosynthesis
DNA, Satellite - genetics
G-box motif
Geminiviridae
gene expression
Genes, Reporter
Glucuronidase - biosynthesis
Glucuronidase - genetics
Host-Pathogen Interactions
Molecular Sequence Data
mutation
Nicotiana - virology
Nicotiana tabacum
Plant Diseases - virology
promoter regions
Promoter Regions, Genetic
Protein Binding
reporter genes
satellite DNA
Sequence Deletion
tobacco
Transcription, Genetic
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNA β is a circular single-stranded satellite DNA associated with certain monopartite begomoviruses (family Geminiviridae) which causes economically important diseases such as cotton leaf curl disease. DNA β contains a single gene, βC1, which encodes a pathogenicity protein responsible for symptom production. Transient expression studies in Nicotiana tabacum using the β-glucuronidase reporter gene driven by a βC1 promoter-deletion series of the DNA β associated with cotton leaf curl Multan virus identified a 68 nt region (between -139 and -207) which is important for βC1 transcription. This 68 nt region contains a G-box (CACGTG) located 143 nt upstream of the βC1 start codon. Mutation of the G-box resulted in a significant reduction in βC1 promoter activity and DNA β replication efficiency. In addition, the G-box motif was found to bind specifically to a protein(s) in nuclear extracts prepared from tobacco leaf tissues. Our results indicate that interaction of the G-box motif with host nuclear factors is important for efficient gene expression and replication of DNA β.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.002980-0