Loading…

Simultaneous imaging of cerebral partial pressure of oxygen and blood flow during functional activation and cortical spreading depression

We developed a novel imaging technique that provides real-time two-dimensional maps of the absolute partial pressure of oxygen and relative cerebral blood flow in rats by combining phosphorescence lifetime imaging with laser speckle contrast imaging. Direct measurement of blood oxygenation based on...

Full description

Saved in:

Bibliographic Details
Published in:	Applied optics. Optical technology and biomedical optics 2009-04, Vol.48 (10), p.D169
Main Authors:	Sakadzić, Sava, Yuan, Shuai, Dilekoz, Ergin, Ruvinskaya, Svetlana, Vinogradov, Sergei A, Ayata, Cenk, Boas, David A
Format:	Article
Language:	English
Subjects:	Animals Cerebral Cortex - blood supply Cerebrovascular Circulation Cortical Spreading Depression - physiology Diagnostic Imaging - methods Electric Stimulation Forelimb - physiology Laser-Doppler Flowmetry Luminescent Measurements Oxygen - blood Partial Pressure Rats Rats, Sprague-Dawley
Citations:	Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c448t-fbb5b1413e101ed06ebe60fdb0208691e3d72f62d008b27329d70e7e4a2414473
cites
container_end_page
container_issue	10
container_start_page	D169
container_title	Applied optics. Optical technology and biomedical optics
container_volume	48
creator	Sakadzić, Sava Yuan, Shuai Dilekoz, Ergin Ruvinskaya, Svetlana Vinogradov, Sergei A Ayata, Cenk Boas, David A
description	We developed a novel imaging technique that provides real-time two-dimensional maps of the absolute partial pressure of oxygen and relative cerebral blood flow in rats by combining phosphorescence lifetime imaging with laser speckle contrast imaging. Direct measurement of blood oxygenation based on phosphorescence lifetime is not significantly affected by changes in the optical parameters of the tissue during the experiment. The potential of the system as a novel tool for quantitative analysis of the dynamic delivery of oxygen to support brain metabolism was demonstrated in rats by imaging cortical responses to forepaw stimulation and the propagation of cortical spreading depression waves. This new instrument will enable further study of neurovascular coupling in normal and diseased brain.
doi_str_mv	10.1364/ao.48.00d169
format	article
fullrecord	<record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_19340106</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19340106</sourcerecordid><originalsourceid>FETCH-LOGICAL-c448t-fbb5b1413e101ed06ebe60fdb0208691e3d72f62d008b27329d70e7e4a2414473</originalsourceid><addsrcrecordid>eNo1kMtOwzAQRS0kREthxxr5B1LGjzjJElW8pEosgHVlx-MqKIkjOwH6Cfw1ToHVnce9R5oh5IrBmgklb7Rfy3INYJmqTsiSszzPBFP5gpzH-A4gclkVZ2TBKiGBgVqS75emm9pR9-inSJtO75t-T72jNQY0Qbd00GFsZg0Y4xRwXvqvwx57qntLTeu9pa71n9ROYQ67qa_Hxvcpo1Pxoefm6K19QtVpHhNM29ls8chNjgty6nQb8fJPV-Tt_u5185htnx-eNrfbrJayHDNnTG6YZAIZMLSg0KACZw1wKFXFUNiCO8UtQGl4IXhlC8ACpeaSSVmIFbn-5Q6T6dDuhpCuDofd_0_ED30UZM0</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Simultaneous imaging of cerebral partial pressure of oxygen and blood flow during functional activation and cortical spreading depression</title><source>OSA Publishing</source><creator>Sakadzić, Sava ; Yuan, Shuai ; Dilekoz, Ergin ; Ruvinskaya, Svetlana ; Vinogradov, Sergei A ; Ayata, Cenk ; Boas, David A</creator><creatorcontrib>Sakadzić, Sava ; Yuan, Shuai ; Dilekoz, Ergin ; Ruvinskaya, Svetlana ; Vinogradov, Sergei A ; Ayata, Cenk ; Boas, David A</creatorcontrib><description>We developed a novel imaging technique that provides real-time two-dimensional maps of the absolute partial pressure of oxygen and relative cerebral blood flow in rats by combining phosphorescence lifetime imaging with laser speckle contrast imaging. Direct measurement of blood oxygenation based on phosphorescence lifetime is not significantly affected by changes in the optical parameters of the tissue during the experiment. The potential of the system as a novel tool for quantitative analysis of the dynamic delivery of oxygen to support brain metabolism was demonstrated in rats by imaging cortical responses to forepaw stimulation and the propagation of cortical spreading depression waves. This new instrument will enable further study of neurovascular coupling in normal and diseased brain.</description><identifier>EISSN: 2155-3165</identifier><identifier>DOI: 10.1364/ao.48.00d169</identifier><identifier>PMID: 19340106</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Cerebral Cortex - blood supply ; Cerebrovascular Circulation ; Cortical Spreading Depression - physiology ; Diagnostic Imaging - methods ; Electric Stimulation ; Forelimb - physiology ; Laser-Doppler Flowmetry ; Luminescent Measurements ; Oxygen - blood ; Partial Pressure ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Applied optics. Optical technology and biomedical optics, 2009-04, Vol.48 (10), p.D169</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-fbb5b1413e101ed06ebe60fdb0208691e3d72f62d008b27329d70e7e4a2414473</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19340106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakadzić, Sava</creatorcontrib><creatorcontrib>Yuan, Shuai</creatorcontrib><creatorcontrib>Dilekoz, Ergin</creatorcontrib><creatorcontrib>Ruvinskaya, Svetlana</creatorcontrib><creatorcontrib>Vinogradov, Sergei A</creatorcontrib><creatorcontrib>Ayata, Cenk</creatorcontrib><creatorcontrib>Boas, David A</creatorcontrib><title>Simultaneous imaging of cerebral partial pressure of oxygen and blood flow during functional activation and cortical spreading depression</title><title>Applied optics. Optical technology and biomedical optics</title><addtitle>Appl Opt</addtitle><description>We developed a novel imaging technique that provides real-time two-dimensional maps of the absolute partial pressure of oxygen and relative cerebral blood flow in rats by combining phosphorescence lifetime imaging with laser speckle contrast imaging. Direct measurement of blood oxygenation based on phosphorescence lifetime is not significantly affected by changes in the optical parameters of the tissue during the experiment. The potential of the system as a novel tool for quantitative analysis of the dynamic delivery of oxygen to support brain metabolism was demonstrated in rats by imaging cortical responses to forepaw stimulation and the propagation of cortical spreading depression waves. This new instrument will enable further study of neurovascular coupling in normal and diseased brain.</description><subject>Animals</subject><subject>Cerebral Cortex - blood supply</subject><subject>Cerebrovascular Circulation</subject><subject>Cortical Spreading Depression - physiology</subject><subject>Diagnostic Imaging - methods</subject><subject>Electric Stimulation</subject><subject>Forelimb - physiology</subject><subject>Laser-Doppler Flowmetry</subject><subject>Luminescent Measurements</subject><subject>Oxygen - blood</subject><subject>Partial Pressure</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>2155-3165</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNo1kMtOwzAQRS0kREthxxr5B1LGjzjJElW8pEosgHVlx-MqKIkjOwH6Cfw1ToHVnce9R5oh5IrBmgklb7Rfy3INYJmqTsiSszzPBFP5gpzH-A4gclkVZ2TBKiGBgVqS75emm9pR9-inSJtO75t-T72jNQY0Qbd00GFsZg0Y4xRwXvqvwx57qntLTeu9pa71n9ROYQ67qa_Hxvcpo1Pxoefm6K19QtVpHhNM29ls8chNjgty6nQb8fJPV-Tt_u5185htnx-eNrfbrJayHDNnTG6YZAIZMLSg0KACZw1wKFXFUNiCO8UtQGl4IXhlC8ACpeaSSVmIFbn-5Q6T6dDuhpCuDofd_0_ED30UZM0</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Sakadzić, Sava</creator><creator>Yuan, Shuai</creator><creator>Dilekoz, Ergin</creator><creator>Ruvinskaya, Svetlana</creator><creator>Vinogradov, Sergei A</creator><creator>Ayata, Cenk</creator><creator>Boas, David A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20090401</creationdate><title>Simultaneous imaging of cerebral partial pressure of oxygen and blood flow during functional activation and cortical spreading depression</title><author>Sakadzić, Sava ; Yuan, Shuai ; Dilekoz, Ergin ; Ruvinskaya, Svetlana ; Vinogradov, Sergei A ; Ayata, Cenk ; Boas, David A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-fbb5b1413e101ed06ebe60fdb0208691e3d72f62d008b27329d70e7e4a2414473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Cerebral Cortex - blood supply</topic><topic>Cerebrovascular Circulation</topic><topic>Cortical Spreading Depression - physiology</topic><topic>Diagnostic Imaging - methods</topic><topic>Electric Stimulation</topic><topic>Forelimb - physiology</topic><topic>Laser-Doppler Flowmetry</topic><topic>Luminescent Measurements</topic><topic>Oxygen - blood</topic><topic>Partial Pressure</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakadzić, Sava</creatorcontrib><creatorcontrib>Yuan, Shuai</creatorcontrib><creatorcontrib>Dilekoz, Ergin</creatorcontrib><creatorcontrib>Ruvinskaya, Svetlana</creatorcontrib><creatorcontrib>Vinogradov, Sergei A</creatorcontrib><creatorcontrib>Ayata, Cenk</creatorcontrib><creatorcontrib>Boas, David A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Applied optics. Optical technology and biomedical optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakadzić, Sava</au><au>Yuan, Shuai</au><au>Dilekoz, Ergin</au><au>Ruvinskaya, Svetlana</au><au>Vinogradov, Sergei A</au><au>Ayata, Cenk</au><au>Boas, David A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous imaging of cerebral partial pressure of oxygen and blood flow during functional activation and cortical spreading depression</atitle><jtitle>Applied optics. Optical technology and biomedical optics</jtitle><addtitle>Appl Opt</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>48</volume><issue>10</issue><spage>D169</spage><pages>D169-</pages><eissn>2155-3165</eissn><abstract>We developed a novel imaging technique that provides real-time two-dimensional maps of the absolute partial pressure of oxygen and relative cerebral blood flow in rats by combining phosphorescence lifetime imaging with laser speckle contrast imaging. Direct measurement of blood oxygenation based on phosphorescence lifetime is not significantly affected by changes in the optical parameters of the tissue during the experiment. The potential of the system as a novel tool for quantitative analysis of the dynamic delivery of oxygen to support brain metabolism was demonstrated in rats by imaging cortical responses to forepaw stimulation and the propagation of cortical spreading depression waves. This new instrument will enable further study of neurovascular coupling in normal and diseased brain.</abstract><cop>United States</cop><pmid>19340106</pmid><doi>10.1364/ao.48.00d169</doi><oa>free_for_read</oa></addata></record>
fulltext	fulltext
identifier	EISSN: 2155-3165
ispartof	Applied optics. Optical technology and biomedical optics, 2009-04, Vol.48 (10), p.D169
issn	2155-3165
language	eng
recordid	cdi_pubmed_primary_19340106
source	OSA Publishing
subjects	Animals Cerebral Cortex - blood supply Cerebrovascular Circulation Cortical Spreading Depression - physiology Diagnostic Imaging - methods Electric Stimulation Forelimb - physiology Laser-Doppler Flowmetry Luminescent Measurements Oxygen - blood Partial Pressure Rats Rats, Sprague-Dawley
title	Simultaneous imaging of cerebral partial pressure of oxygen and blood flow during functional activation and cortical spreading depression
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T20%3A57%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20imaging%20of%20cerebral%20partial%20pressure%20of%20oxygen%20and%20blood%20flow%20during%20functional%20activation%20and%20cortical%20spreading%20depression&rft.jtitle=Applied%20optics.%20Optical%20technology%20and%20biomedical%20optics&rft.au=Sakadzi%C4%87,%20Sava&rft.date=2009-04-01&rft.volume=48&rft.issue=10&rft.spage=D169&rft.pages=D169-&rft.eissn=2155-3165&rft_id=info:doi/10.1364/ao.48.00d169&rft_dat=%3Cpubmed%3E19340106%3C/pubmed%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c448t-fbb5b1413e101ed06ebe60fdb0208691e3d72f62d008b27329d70e7e4a2414473%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/19340106&rfr_iscdi=true