Transient elevation of neutrophil proteinases in induced sputum during COPD exacerbation

Objective. Patients with chronic obstructive pulmonary disease (COPD) are prone to acute exacerbations associated with increased morbidity and mortality. One potential group of enzymes causing tissue destruction in this disease includes neutrophil proteinase elastase (NE), collagenase-2 (matrix meta...

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Bibliographic Details
Published in:Scandinavian journal of clinical and laboratory investigation 2008, Vol.68 (7), p.618-623
Main Authors: Ilumets, Helen, Rytilä, Paula H., Sovijärvi, Anssi R., Tervahartiala, Taina, Myllärniemi, Marjukka, Sorsa, Timo A., Kinnula, Vuokko L.
Format: Article
Language:English
Subjects:
Aged
COPD
Female
Forced Expiratory Volume - physiology
Humans
Leukocyte Elastase - metabolism
Male
Matrix Metalloproteinase 8 - metabolism
Matrix Metalloproteinase 9 - metabolism
Middle Aged
MMP-8
MMP-9
neutrophil elastase
Neutrophils - enzymology
Pulmonary Disease, Chronic Obstructive - enzymology
Respiratory Function Tests
Smoking - physiopathology
sputum
Sputum - cytology
Sputum - enzymology
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Summary:Objective. Patients with chronic obstructive pulmonary disease (COPD) are prone to acute exacerbations associated with increased morbidity and mortality. One potential group of enzymes causing tissue destruction in this disease includes neutrophil proteinase elastase (NE), collagenase-2 (matrix metalloproteinase-8 (MMP-8)) and gelatinase B (MMP-9). We investigated the activity of NE and the levels of MMP-8 and MMP-9 in a longitudinal setting at and after COPD exacerbation using a non-invasive technique, i.e. induced sputum, to ascertain whether these proteinases play a role in COPD exacerbation. Material and methods. The study included healthy non-smokers (n = 32), healthy smokers (n = 28), patients with stable COPD (n = 15), COPD patients with acute exacerbations (exa) (n = 10) and their recovery (n = 8) after 4 weeks. NE activity by synthetic peptide substrate and spectrophotometry, MMP-8 levels by immunofluorometry and MMP-9 levels by ELISA were analysed from induced sputum supernatants. Results. NE activity and the level of MMP-8 increased highly significantly in patients with COPD exacerbation compared to stable COPD and controls (NE: p = 0.001 and p
ISSN:0036-5513
1502-7686
DOI:10.1080/00365510801983773